Somatic control over the germline stem cell lineage during Drosophila spermatogenesis
Somatic control over the germline stem cell lineage during Drosophila spermatogenesis
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DOI：
10.1038/35037613
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发表时间：
2000-10-12
期刊：
影响因子：
64.8
通讯作者：
DiNardo, S
中科院分区：
文献类型：
--
作者：
Tran, J;Brenner, TJ;DiNardo, S
干细胞分裂既能产生新的干细胞，也能产生能够分化的子细胞。潜在的机制尚不清楚，但从概念上讲有两种(2)。内在机制可能控制决定因素的不平等分裂，导致不对称细胞分裂，产生一个干细胞和一个分化的子细胞。或者，外部机制，包括基质细胞信号，可能导致子细胞保持在适当的生态位，而离开这个生态位的子细胞分化。在这里，我们使用果蝇精子发生作为模型干细胞系统(3)来证明在缺乏Raf活性的睾丸中存在过量的干细胞和性腺母细胞。此外，生殖系干细胞群体保持活跃的寿命比野生型更长。最后，环绕生殖细胞的体细胞也需要raf。我们得出结论，细胞外源性机制调节生殖系干细胞的行为。
Stem cells divide both to produce new stem cells and to generate daughter cells that can differentiate(1). The underlying mechanisms are not well understood, but conceptually are of two kinds(2). Intrinsic mechanisms may control the unequal partitioning of determinants leading to asymmetric cell divisions that yield one stem cell and one differentiated daughter cell. Alternatively, extrinsic mechanisms, involving stromal cell signals, could cause daughter cells that remain in their proper niche to stay stem cells, whereas daughter cells that leave this niche differentiate. Here we use Drosophila spermatogenesis as a model stem cell system(3) to show that there are excess stem cells and gonialblasts in testes that are deficient for Raf activity. In addition, the germline stem cell population remains active for a longer fraction of lifespan than in wild type. Finally, raf is required in somatic cells that surround germ cells. We conclude that a cell-extrinsic mechanism regulates germline stem cell behaviour.