A novel C1q domain containing protein in black rockfish (Sebastes schlegelii) serves as a pattern recognition receptor with immunoregulatory properties and possesses binding activity to heat-aggregated IgG
A novel C1q domain containing protein in black rockfish (Sebastes schlegelii) serves as a pattern recognition receptor with immunoregulatory properties and possesses binding activity to heat-aggregated IgG
复制标题
黑石斑鱼 (Sebastes schlegelii) 中含有新型 C1q 结构域的蛋白质可作为具有免疫调节特性的模式识别受体，并具有与热聚集 IgG 的结合活性
DOI：
10.1016/j.fsi.2019.01.004
复制
发表时间：
2019-04-01
影响因子：
4.7
通讯作者：
Hu, Yong-hua
中科院分区：
文献类型：
--
作者：
Du, Xue;Wang, Guang-hua;Hu, Yong-hua
C1 qDC蛋白是脊椎动物和无脊椎动物天然免疫中重要的模式识别受体，参与一系列免疫应答。C1 qDC蛋白在脊椎动物中的功能研究很少。在本研究中，C1 qDC蛋白（SsC 1 qDC）从硬骨鱼黑岩鱼（Schlegelii）的鉴定和研究在表达和功能水平。SsC 1 qDC的开放阅读框为636 bp，其氨基酸序列与几种鱼类的C1 qDC蛋白的同源性为62%~ 69%. SsC 1 qDC具有保守的C1 qDC特征，包括信号序列和C1 q结构域。SsClqDC在不同组织中表达，并且其表达被细菌和病毒感染上调。重组SsC 1 qDC（rSsC 1 qDC）对PAMPs（包括LPS和PGN）具有明显的结合活性。rSsC 1 qDC对副溶血性弧菌具有抗菌活性，并能增强巨噬细胞对鳗弧菌的吞噬活性。rSsC 1 qDC与人热聚集IgG相互作用。此外，在rSsC 1 qDC的存在下，鱼表现出增强的抵抗细菌感染。这些结果表明，SsC 1 qDC作为一种模式识别受体，在黑岩鱼的防御系统中起着重要作用。
C1q-domain-containing (C1qDC) proteins, which are involved in a series of immune responses, are important pattern recognition receptors in innate immunity in vertebrates and invertebrates. Functional studies of C1qDC proteins in vertebrates are scarce. In the present study, a C1qDC protein (SsC1qDC) from the teleost black rockfish (Sebastes schlegelii) was identified and examined at expression and functional levels. The open reading frame of SsC1qDC is 636 bp, and the predicted amino acid sequence of SsC1qDC shares 62%-69% overall identity with the C1qDC proteins of several fish species. SsC1qDC possesses conserved C1qDC features, including a signal sequence and a C1q domain. SsClqDC was expressed in different tissues and its expression was upregulated by bacterial and viral infection. Recombinant SsC1qDC (rSsC1qDC) exhibited apparent binding activities against PAMPs including LPS and PGN. rSsC1qDC had antibacterial activity against Vibrio parehaemolyticus, and was able to enhance the phagocytic activity of macrophages towards Vibrio anguillarwn. rSsC1qDC interacted with human heat-aggregated IgG. Furthermore, in the presence of rSsC1qDC, fish exhibited enhanced resistance against bacterial infection. Collectively, these results indicated that SsC1qDC serves as a pattern recognition receptor and plays a vital role in the defense system of black rockfish.