Comprehensive 3D phenotyping reveals continuous morphological variation across genetically diverse sorghum inflorescences
Comprehensive 3D phenotyping reveals continuous morphological variation across genetically diverse sorghum inflorescences
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DOI：
10.1111/nph.16533
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发表时间：
2020-04-16
期刊：
影响因子：
9.4
通讯作者：
Topp, Christopher N.
中科院分区：
文献类型：
--
作者：
Li, Mao;Shao, Mon-Ray;Topp, Christopher N.
植物中的花序结构通常很复杂且挑战，特别是对于谷物草的花序。捕获花序结构和分析所得数据的方法仅限于一些易于捕获的参数，这些参数可能会错过富裕的潜在多样性。在这里，我们应用X射线计算机断层扫描与详细的形态计量学结合，提供新的成像和计算工具来分析三维的花序结构。为了展示这种方法的力量，我们专注于高粱双色的销片，它们在主要分支的数量，长度和角度各不相同，以及种子的三维形状，大小和分布。我们成像并全面地评估了55个综合式涂层的综合分类的综合分类，以固有分类为基因研究。我们使用数据来确定形态学特征分配标本进行比赛的可靠性，并发现种子特征特别有用。黑色Circleblack Circleblack Circleblack Circle
Inflorescence architecture in plants is often complex and challenging to quantify, particularly for inflorescences of cereal grasses. Methods for capturing inflorescence architecture and for analyzing the resulting data are limited to a few easily captured parameters that may miss the rich underlying diversity.Here, we apply X-ray computed tomography combined with detailed morphometrics, offering new imaging and computational tools to analyze three-dimensional inflorescence architecture. To show the power of this approach, we focus on the panicles of Sorghum bicolor, which vary extensively in numbers, lengths, and angles of primary branches, as well as the three-dimensional shape, size, and distribution of the seed.We imaged and comprehensively evaluated the panicle morphology of 55 sorghum accessions that represent the five botanical races in the most common classification system of the species, defined by genetic data. We used our data to determine the reliability of the morphological characters for assigning specimens to race and found that seed features were particularly informative.However, the extensive overlap between botanical races in multivariate trait space indicates that the phenotypic range of each group extends well beyond its overall genetic background, indicating unexpectedly weak correlation between morphology, genetic identity, and domestication history. BLACK CIRCLEBLACK CIRCLEBLACK CIRCLEBLACK CIRCLE