Mss51 and Ssc1 Facilitate Translational Regulation of Cytochrome c Oxidase Biogenesis
Mss51 and Ssc1 Facilitate Translational Regulation of Cytochrome c Oxidase Biogenesis
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DOI：
10.1128/mcb.00983-09
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发表时间：
2010-01-01
影响因子：
5.3
通讯作者：
Barrientos, Antoni
中科院分区：
文献类型：
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作者：
Fontanesi, Flavia;Soto, Iliana C.;Barrientos, Antoni
复杂的生物合成的多聚体细胞器酶的双重遗传起源需要几个层次的调节。在酿酒酵母中，线粒体细胞色素c氧化酶（考克斯）的组装是受调控的。亚基1（Cox 1）的合成取决于其组装伙伴的可用性，从而充当负反馈环，在考克斯组装期间协调COX 1 mRNA翻译与Cox 1利用。COX 1 mRNA特异性翻译激活因子Mss 51在这一过程中起着重要作用。在这里，我们报告说，Mss 51连续相互作用的COX 1 mRNA的翻译装置，新合成的COX 1，和其他考克斯组装因子在COX 1成熟/组装。值得注意的是，线粒体Hsp 70分子伴侣Ssc 1被证明是Mss 51的整个代谢周期的合作伙伴。我们的结论是，Ssc 1，通过与Mss 51和Mss 51-含有复合物，在Cox 1的生物合成，考克斯组装，和这些过程的翻译调节中起着至关重要的作用。
The intricate biogenesis of multimeric organellar enzymes of dual genetic origin entails several levels of regulation. In Saccharomyces cerevisiae, mitochondrial cytochrome c oxidase (COX) assembly is regulated translationally. Synthesis of subunit 1 (Cox1) is contingent on the availability of its assembly partners, thereby acting as a negative feedback loop that coordinates COX1 mRNA translation with Cox1 utilization during COX assembly. The COX1 mRNA-specific translational activator Mss51 plays a fundamental role in this process. Here, we report that Mss51 successively interacts with the COX1 mRNA translational apparatus, newly synthesized Cox1, and other COX assembly factors during Cox1 maturation/assembly. Notably, the mitochondrial Hsp70 chaperone Ssc1 is shown to be an Mss51 partner throughout its metabolic cycle. We conclude that Ssc1, by interacting with Mss51 and Mss51-containing complexes, plays a critical role in Cox1 biogenesis, COX assembly, and the translational regulation of these processes.