The Anopheles stephensi odorant binding protein 1 (AsteObp1) gene: A new molecular marker for biological forms diagnosis
The Anopheles stephensi odorant binding protein 1 (AsteObp1) gene: A new molecular marker for biological forms diagnosis
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DOI：
10.1016/j.actatropica.2015.03.012
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发表时间：
2015-06-01
期刊：
影响因子：
2.7
通讯作者：
Raz, A.
中科院分区：
文献类型：
--
作者：
Gholizadeh, S.;Firooziyan, S.;Raz, A.
斯氏按蚊1901是已知的亚洲疟疾病媒。三种生物形态，即“mysorensis”，“intermediate”和“type”已在该物种中报道过。然而，目前的形态学和分子信息不足以诊断这些形式。在调查过程中，安。对斯蒂芬氏菌的生物形态进行了形态学鉴定，并对气味结合蛋白1 （Obp1）基因进行了测序。此外，内含子I序列用于构建系统发育树。尽管AsteObp1外显子的核苷酸序列存在差异，但在氨基酸水平上，三种生物形式的同源性接近100%。为了克服利用卵细胞形态特征的困难，利用卵的内含子I序列。斯氏杆菌Obp1为鉴定亚洲主要疟疾媒介生物形式开辟了新的分子途径。但尚需多学科研究来确定其分类学地位。stephensi。(C) 2015 Elsevier B.V.版权所有
Anopheles (Cellia) stephensi Liston 1901 is known as an Asian malaria vector. Three biological forms, namely "mysorensis", "intermediate", and "type" have been earlier reported in this species. Nevertheless, the present morphological and molecular information is insufficient to diagnose these forms. During this investigation, An. stephensi biological forms were morphologically identified and sequenced for odorant-binding protein 1 (Obp1) gene. Also, intron I sequences were used to construct phylogenetic trees. Despite nucleotide sequence variation in exon of AsteObp1, nearly 100% identity was observed at the amino acid level among the three biological forms. In order to overcome difficulties in using egg morphology characters, intron I sequences of An. stephensi Obp1 opens new molecular way to the identification of the main Asian malaria vector biological forms. However, multidisciplinary studies are needed to establish the taxonomic status of An. stephensi. (C) 2015 Elsevier B.V. All rights reserved.