Klebsiella pneumoniae lipopolysaccharide O typing:: Revision of prototype strains and O-group distribution among clinical isolates from different sources and countries
Klebsiella pneumoniae lipopolysaccharide O typing:: Revision of prototype strains and O-group distribution among clinical isolates from different sources and countries
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DOI：
10.1128/jcm.37.1.56-62.1999
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发表时间：
1999-01-01
影响因子：
9.4
通讯作者：
Benedí, VJ
中科院分区：
文献类型：
--
作者：
Hansen, DS;Mestre, F;Benedí, VJ
我们之前已经描述了一种抑制酶联免疫吸附法用于肺炎克雷伯菌O1脂多糖的O分型，该方法克服了经典O分型方法的技术问题和局限性。在本研究中，我们将该方法扩展到目前已知的所有O型。通过研究用经典的管凝集O型方法定义了O群的原型菌株，验证了该方法的有效性。根据这些结果，我们确定了64株可分型菌株中60株的O型，并提出了一种修改后的O型抗原方案，该方案进行了微小但必要的修改，包括O1、O2、O2ac、O3、O4、O5、O7、O8和O12血清型。对来自丹麦、西班牙和美国不同来源（血液、尿液和其他）的638株肺炎克雷伯菌临床分离株的分型方法应用表明，这些分离株中高达80%属于O1、O2、O3和O5血清型或血清组，与分离源无关，并且主要的不可分型分离株（约占总数的17%）由一半的O+和一半的O-菌株组成。然而，根据国家和分离来源的不同，在脂多糖O型或组的流行率方面观察到差异。
We have previously described an inhibition enzyme-linked immunosorbent assay method for the O typing of O1 lipopolysaccharide from Klebsiella pneumoniae which overcomes the technical problems end limitations of the classical O-typing method. In this study, we have extended the method to all of the currently recognized O types. The method was validated by studying the prototype strains that have defined the O groups by the classical tube agglutinatination O-typing method. Based on these results, we confirmed the O types of 60 of 64 typeable strains, and we propose a revised O-antigenic scheme, with minor but necessary changes, consisting of serogroups or serotypes O1, O2, O2ac, O3, O4, O5, O7, O8, and O12. Application of this typing method to 638 K. pneumoniae clinical isolates from Denmark, Spain, and the United States from different sources (blood, urine, and others) showed that up to 80% of these isolates belong to serotypes or serogroups O1, O2, O3, and O5, independently of the source of isolation, and that a major group of nontypeable isolates, representing about 17% of the total, consists of half O+ and half O- strains. Differences were observed, however, in the prevalence of the lipopolysaccharide O types or groups, depending on the country and isolation source.