Burkholderia oxyphila sp. nov., a bacterium isolated from acidic forest soil that catabolizes (+)-catechin and its putative aromatic derivatives
Burkholderia oxyphila sp. nov., a bacterium isolated from acidic forest soil that catabolizes (+)-catechin and its putative aromatic derivatives
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DOI：
10.1099/ijs.0.017368-0
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发表时间：
2011-02-01
影响因子：
2.8
通讯作者：
Murata, Hitoshi
中科院分区：
文献类型：
--
作者：
Otsuka, Yuichiro;Muramatsu, Yuki;Murata, Hitoshi
从酸性土壤中分离到一株新的细菌OX-01(T)，对其进行了分类鉴定，鉴定为一种能将(+)-儿茶素分解为紫杉酯的细菌。OX-01(T)是一株革兰氏反应阴性、需氧、不产孢子、不能运动的杆状细菌。16S rRNA基因序列分析表明，该菌株属于伯克霍尔德氏菌属，其系统发育位置与甘蔗伯克霍尔德氏菌最接近，但明显不同。OX-01(T)菌株在其基因组中没有任何固氮所必需的近等位基因，这一特征类似于缺乏nifH的甘蔗芽孢杆菌，但有别于许多其他系统发育相关的分类群的固氮特征，如铁伯克霍尔德氏菌、B.heleia、含羞草杆菌、结瘤B.nodosa、B.silvatlantica、B.tropica和B.unamae。菌株OX-01(T)的化学分类特征为：主要泛酚为Q-8，DNAG+C含量为摩尔%，主要脂肪酸为C-16：0、C-17：0环和C-18：1欧米伽7c。在伯克霍尔德氏菌属的其他物种中，它也有独特的碳水化合物利用概况。该菌株不能同化许多戊糖、己糖和低聚糖，但能分解(+)-儿茶素及其可能的芳香族衍生物，如4-羟基-3-甲氧基肉桂酸、原儿茶酸、对羟基苯甲酸、反式-对香豆酸和香草酸。根据其形态、生理和化学分类特征，结合DNA-DNA亲缘关系值和16S rRNA基因序列比对数据，我们认为OX-O1(T)菌株是伯克霍尔德氏菌属的一个新种，命名为Burkholderia oxPhila sp.11月是提议的。模式菌株为OX-01(T)(=NBRC 105797(T)=DSM 22550(T))。
A novel bacterium, designated strain OX-01(T), was isolated from acidic soil, taxonomically investigated and identified as an agent that catabolizes (+)-catechin into taxifolin. Strain OX-01(T) is a Gram-reaction-negative, aerobic, non-sporulating, non-motile and rod-shaped bacterium. 16S rRNA gene sequence analysis identified this strain as a member of the genus Burkholderia and occupying a phylogenetic position closest to, but clearly distinct from, Burkholderia sacchari. Strain OX-01(T) does not have any nil genes, which are required for N-2-fixation, in its genome, a feature that is similar to B. sacchari, which lacks nifH, but is distinct from the N-2-fixing features of many other phylogenetically related taxa, such as Burkholderia ferrariae, B. heleia, B. mimosarum, B. nodosa, B. silvatlantica, B. tropica and B. unamae. Strain OX-01(T) has the following chemotaxonomic characteristics: the major ubiquinone is Q-8, the DNA G + C content is 64 mol% and the major fatty acids are C-16:0, C-17:0 cyclo and C-18:1 omega 7c. It also has a unique profile of carbohydrate utilization among other species of the genus Burkholderia. The strain cannot assimilate many pentoses, hexoses and oligosaccharides, whereas it can catabolize (+)-catechin and its putative aromatic derivatives, such as 4-hydroxy-3-methoxycinnamic acid, protocatechuic acid, p-hydroxybenzoic acid, trans-p-coumaric acid and vanillic acid. Based on its morphological, physiological and chemotaxonomic characteristics, together with DNA-DNA relatedness values and 16S rRNA gene sequence comparison data, we show that strain OX-O1(T) represents a novel species of the genus Burkholderia, for which the name Burkholderia oxyphila sp. nov. is proposed. The type strain is OX-01(T) (=NBRC 105797(T) =DSM 22550(T)).