Studies on the culturable marine actinomycetes isolated from the Nahoon beach in the Eastern Cape Province of South Africa
Studies on the culturable marine actinomycetes isolated from the Nahoon beach in the Eastern Cape Province of South Africa
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南非东开普省 Nahoon 海滩分离的可培养海洋放线菌研究
DOI：
10.5897/ajmr.9000672
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发表时间：
2010
影响因子：
--
通讯作者：
A. Okoh
中科院分区：
文献类型：
--
作者：
I. N. Ogunmwonyi;N. Mazomba;L. Mabinya;E. Ngwenya;E. Green;D. Akinpelu;A. Olaniran;Kim Bernard;A. Okoh
从南非东开普省印度洋海岸Nahoon海滩的水和岩石碎屑中分离出84种可培养的海洋放线菌，属于15种一般形态。菌落形态特征多样，其发生和分布随样品类型的不同而不同。岩石碎屑比水样产生更多的放线菌形态。链霉菌在两个样本中最为普遍。岩石碎屑中存在放线素平面、放线素系、Norcadia、Norcardiopsis、Streptosporagium和Intrasporagium，而水样中不存在放线素平面、放线素系。水样中存在基带孢子菌，岩石碎屑中不存在基带孢子菌。对分离的链霉菌进行了初步的抗菌活性筛选。用等体积的乙酸乙酯（1:1 v/v）提取10株最强效链霉菌分离物2次。推定链霉菌分离物的乙酸乙酯提取物对筛选的32种测试细菌中的至少6种至26种具有活性。在浓度为10 mg/ml时，发现抑制区直径在9至32 mm之间。通过红外光谱分析对粗提物进行了部分表征，发现粗提物中可能含有萜类、长链脂肪酸和仲胺类衍生物。我们认为，Nahoon海滩有望成为具有生物活性化合物生产和新药发现潜力的各种海洋放线菌的名副其实的资源。
A total of 84 culturable marine actinomycetes belonging to 15 generic morphotypes were isolated from water and rock scrapings from the Nahoon beach, a coastal shore of Indian Ocean in the Eastern Cape Province of South Africa. The colonies showed diverse morphological characteristics and their occurrence and distribution varied with sample type. The rock scrappings yielded more actinomycetes morphotypes than the water samples. Streptomyces was most prevalent in both samples.Actinoplane, Actinosynnema, Norcadia, Norcardiopsis, Streptosporagium and Intrasporagium were present in rock scrapings but absent in water samples. Kibdellosporium was present in water sample and absent in rock scrapings. The Streptomyces isolates were selected and preliminarily screened for antimicrobial activity. The bioactive compounds of 10 most potent Streptomyces isolate was extracted twice with equal volume of ethyl acetate (1:1 v/v). The ethyl acetate extracts of the putative Streptomyces isolates showed activities against at least 6 and up to 26 of the 32 test bacteria screened. Inhibition zones were found to range between 9 and 32 mm diameters at a concentration of 10 mg/ml. The partial characterization of the crude extracts by IR spectra analysis revealed the possible presence of terpenoid, long chain fatty acids and secondary amine derivatives compounds in the extracts. We conclude that the Nahoon beach promises to be a veritable resource for diverse marine actinomycetes of potentials for bioactive compounds production and novel drug discovery.
Key words: Morphotypes, occurrence, rock scrapings, IR spectral, bioactive compound.