Effects of gaseous atmosphere and antioxidants on the development and cryotolerance of bovine embryos at different periods of in vitro culture
Effects of gaseous atmosphere and antioxidants on the development and cryotolerance of bovine embryos at different periods of in vitro culture
复制标题
DOI：
10.1017/s0967199413000361
复制
发表时间：
2015-04-01
期刊：
影响因子：
1.7
通讯作者：
Mingoti, Gisele Zoccal
中科院分区：
文献类型：
--
作者：
de Souza Rocha-Frigoni, Nathalia Alves;da Silva Leao, Beatriz Caetano;Mingoti, Gisele Zoccal
本研究考察了添加抗氧化剂和O-2张力对胚胎发育、低温耐受性和细胞内活性氧（ROS）水平的影响。抗氧化剂添加量为0.6 mM半胱氨酸（囊肿）；0.6 mM半胱氨酸+ 100 μ M半胱氨酸（C+C）；100 IU过氧化氢酶（CAT）或100 μ M -巯基乙醇（β -me）进行3或7天的体外培养（IVC）。测试了两种O-2张力（20% O-2[空气中5% CO2]或7% O-2, 5% CO2和88% N-2[气体混合物]）。添加抗氧化剂7 d后，各处理对囊胚频率均有不利影响（P 0.05）（范围为66% ~ 100%）。添加抗氧化剂3 d后，任何一种抗氧化剂（43.6 ~ 48.5%）均未影响囊胚频率（P < 0.05），但抗氧化剂处理（0.78 ~ 0.95）或O-2浓度（20%和7% O-2浓度分别为0.86和0.88）降低了囊胚频率（P < 0.05）。再膨胀率不受任何处理的影响（P < 0.05）（范围为63.6 ~ 93.3%）。综上所述，整个IVC期细胞内添加抗氧化剂和低氧-2张力对胚胎发育有害。然而，直到IVC第3天，抗氧化剂的补充并没有影响囊胚频率或细胞内ROS水平。
This study examined the effects of antioxidant supplementation and O-2 tension on embryo development, cryotolerance and intracellular reactive oxygen species (ROS) levels. The antioxidant supplementation consisted of 0.6 mM cysteine (CYST); 0.6 mM cysteine + 100 mu M cysteamine (C+C); 100 IU catalase (CAT) or 100 mu M beta-mercaptoethanol (beta-ME) for 3 or 7 days of in vitro culture (IVC). Two O-2 tensions (20% O-2 [5% CO2 in air] or 7% O-2, 5% CO2 and 88% N-2 [gaseous mixture]) were examined. After 7 days of antioxidant supplementation, the blastocyst frequencies were adversely affected (P 0.05) by the treatments (range, 66-100%). After 3 days of antioxidant supplementation, the blastocyst frequencies were not affected (P > 0.05) by any of the antioxidants (range, 43.6-48.5%), but they were reduced by low O-2 tension (P 0.05) by antioxidant treatment (range, 0.78 to 0.95) or by O-2 tension (0.86 and 0.88 for 20% and 7% O-2, respectively). The re-expansion rates were not affected (P > 0.05) by any of the treatments (range, 63.6-93.3%). In conclusion, intracellular antioxidant supplementation and low O-2 tension throughout the entire IVC period were deleterious to embryo development. However, antioxidant supplementation up to day 3 of IVC did not affect the blastocyst frequencies or intracellular ROS levels.