Properties of immobilized MAS1-H108A lipase and its application in the efficient synthesis of n-3 PUFA-rich triacylglycerols
Properties of immobilized MAS1-H108A lipase and its application in the efficient synthesis of n-3 PUFA-rich triacylglycerols
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DOI：
10.1007/s00449-020-02470-x
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发表时间：
2020-11
影响因子：
3.8
通讯作者：
Xiumei Wang;Xiaoxu Zhao;Xiaoli Qin;Zexin Zhao;Bo Yang;Yonghua Wang
中科院分区：
文献类型：
--
作者：
Xiumei Wang;Xiaoxu Zhao;Xiaoli Qin;Zexin Zhao;Bo Yang;Yonghua Wang
首次报道了海洋链霉菌W007菌株MAS1-H108A脂肪酶在XAD1180树脂上的固定化性质及其在合成富含n-3多不饱和脂肪酸(PUFA)的三酰甘油(TAG)中的应用。结果表明，固定化脂肪酶MAS1-H108A的最适温度为70℃，游离脂肪酶MAS1-H108A的最适pH为7.0。与游离脂肪酶相比，固定化MAS1-H108A脂肪酶具有更高的热稳定性。同样有趣的是，固定化MAS1-H108A脂肪酶和游离脂肪酶MAS1-H108A在三酸甘油酯的水解中都没有显示出区域特异性。随后，利用固定化脂肪酶MAS1-H108A和游离脂肪酶MAS1-H108A在真空无溶剂体系中催化n-3多不饱和脂肪酸乙酯的甘油分解和n-3多不饱和脂肪酸与甘油的酯化反应。结果表明，非区域特异性固定化MAS1-H108A脂肪酶能高效合成富含n-3PUFA的TAG，在酯化反应和甘油分解反应中TAG含量分别达到92.07%和76.13%，显著高于游离脂肪酶MAS1-H108A的含量(71.82%和39.62%)。此外，TAG的n-3PUFA组成与衬底相似。这些结果表明，非区域特异性固定化MAS1-H108A脂肪酶是工业化合成富含n-3多不饱和脂肪酸TAG的一种有前景的高效生物催化剂。
This study reports the properties of immobilized MAS1-H108A lipase from marineStreptomyces sp.strain W007 on XAD1180 resin and its application in the synthesis of n-3 polyunsaturated fatty acids (PUFA)-rich triacylglycerols (TAG) for the first time. It was found that the optimal temperature and pH for both immobilized MAS1-H108A lipase and free lipase MAS1-H108A were 70 °C and 7.0, respectively. However, immobilized MAS1-H108A lipase exhibited higher thermostability when compared with free lipase MAS1-H108A. It was also interesting that both immobilized MAS1-H108A lipase and free lipase MAS1-H108A showed no regiospecificity in the hydrolysis of triolein. Subsequently, immobilized MAS1-H108A lipase and free lipase MAS1-H108A were employed to catalyze glycerolysis of n-3 PUFA-rich ethyl esters (EE) and esterification of n-3 PUFA with glycerol under vacuum in the solvent-free system. The results showed that n-3 PUFA-rich TAG were synthesized efficiently by non-regiospecific immobilized MAS1-H108A lipase and TAG contents separately reached 92.07% and 76.13% during the esterification and glycerolysis reactions, which were significantly higher than those (71.82% and 39.62%, respectively) obtained by free lipase MAS1-H108A. Besides, TAG exhibited similar n-3 PUFA composition to the substrate. These findings indicated that non-regiospecific immobilized MAS1-H108A lipase is a promising and efficient biocatalyst for the industrial synthesis of n-3 PUFA-rich TAG.