Intronic enhancement of angiotensin II type 2 receptor transgene expression in vitro and in vivo
Intronic enhancement of angiotensin II type 2 receptor transgene expression in vitro and in vivo
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DOI：
10.1016/j.bbrc.2005.08.035
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发表时间：
2005-10-14
影响因子：
3.1
通讯作者：
Sumners, C
中科院分区：
文献类型：
--
作者：
Li, HW;Gao, YX;Sumners, C
血管紧张素II 2型受体(AT2R)可以影响多种细胞内信号分子和细胞功能。然而，它的生理功能和内含子在其表达调控中的作用还没有很好的确定。我们首次证明AT2R的内含子I和内含子2都能显著增强AT2R的过表达。因此，我们为进一步研究AT2R基因表达调控机制提供了一些新的前提。接下来，我们建立了一种利用含有AT2R两个内含子的AT2R重组腺病毒载体在体外和体内高效地传递这种受体的方法。该载体可能有助于揭示AT2R的生理功能，也可用于AT2R相关的基因治疗。此外，我们还确定了内含子在基因表达盒中的重要作用以及目的基因和报告基因之间表达的不一致性。(C)2005 Elsevier Inc.保留所有权利。
The angiotensin II type 2 receptor (AT2R) can influence a variety of intracellular signaling molecules and cellular functions. However, its physiological functions and the roles of introns in the regulation of its expression have not been well determined. We first demonstrated that both intron I and intron 2 of AT2R could significantly enhance AT2R overexpression. Thus, we have provided some new prerequisites for further studies on the mechanisms that control AT2R gene expression. Next, we established a highly efficient method of delivering this receptor in vitro and in vivo using an AT2R recombinant adenoviral vector containing two introns of the AT2R. The vector may be useful in helping to uncover AT2R physiological functions and also for gene therapy related to AT2R. Moreover, we determined the important role of introns in gene expression cassettes and the inconsistency of expression between the targeted gene and the reporter gene. (c) 2005 Elsevier Inc. All rights reserved.