Whole-genome sequencing to control antimicrobial resistance.
Whole-genome sequencing to control antimicrobial resistance.
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DOI：
10.1016/j.tig.2014.07.003
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发表时间：
2014-09
期刊：
影响因子：
--
通讯作者：
Peacock SJ
中科院分区：
文献类型：
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作者：
Köser CU;Ellington MJ;Peacock SJ
由于测序技术的改进，微生物全基因组测序（WGS）已成为控制抗生素耐药性的核心工具。WGS已被用于开发新型抗生素和诊断测试。WGS一直是监测和研究抗生素耐药性出现的关键。快速WGS有可能用作临床感染控制的工具，在某些情况下，可作为检测耐药性的主要诊断工具。随着测序技术的最新改进，全基因组测序（WGS）有望成为控制抗生素耐药性的重要工具，抗生素耐药性是现代医疗保健的主要威胁。WGS已经在这一领域发现了许多应用，从开发新型抗生素和诊断测试到通过监测和阐明导致耐药性出现和持续的因素对现有药物进行抗生素管理。许多原理验证研究也强调了WGS作为日常感染控制工具的价值，以及对于某些病原体，作为检测抗生素耐药性的主要诊断工具。然而，在大规模引入常规WGS之前，需要开发适当的数据分析平台。
Owing to improvements in sequencing technologies, microbial whole-genome sequencing (WGS) has emerged as a central tool to control antibiotic resistance. WGS has been used to develop novel antibiotics and diagnostic tests. WGS has been key to surveillance and the study of the emergence of antibiotic resistance. Rapid WGS has the potential to be used as a tool for infection control in the clinic and, in some cases, as a primary diagnostic tool to detect resistance. Following recent improvements in sequencing technologies, whole-genome sequencing (WGS) is positioned to become an essential tool in the control of antibiotic resistance, a major threat in modern healthcare. WGS has already found numerous applications in this area, ranging from the development of novel antibiotics and diagnostic tests through to antibiotic stewardship of currently available drugs via surveillance and the elucidation of the factors that allow the emergence and persistence of resistance. Numerous proof-of-principle studies have also highlighted the value of WGS as a tool for day-to-day infection control and, for some pathogens, as a primary diagnostic tool to detect antibiotic resistance. However, appropriate data analysis platforms will need to be developed before routine WGS can be introduced on a large scale.