Rgg influences the expression of multiple regulatory loci to coregulate virulence factor expression in Streptococcus pyogenes
Rgg influences the expression of multiple regulatory loci to coregulate virulence factor expression in Streptococcus pyogenes
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DOI：
10.1128/iai.70.2.762-770.2002
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发表时间：
2002-02-01
影响因子：
3.1
通讯作者：
Musser, JM
中科院分区：
文献类型：
--
作者：
Chaussee, MS;Sylva, GL;Musser, JM
人类病原体化脓性链球菌向细胞壁和细胞外环境分泌许多蛋白质，从而产生毒力。 Rgg 调节多种外蛋白的表达，包括半胱氨酸蛋白酶 (SPE B)、核酸酶 (MF-1)、推定核酸酶 (MF-3) 和自溶素。 Rgg 调节的外蛋白的功能异质性以及基因启动子区域缺乏保守的调节基序表明 Rgg 与其他调节网络相互作用以影响基因表达。 DNA 微阵列用于通过比较化脓性链球菌 NZ131 和同基因衍生物 NZ131 rgg 在指数生长期的全基因组转录谱来检验这一假设。 rgg 突变体中已知和推定的毒力相关基因的转录本更加丰富，包括 emm、scpA、orfX、scl1、hasAB、slo、sagA、ska、speH、grab、mac、mf-1 和 mf-3。 rgg 突变体中 emm、sepA 和 orfX 转录的增加与相应蛋白质产量的增加有关。毒力相关基因表达的差异与一些调控基因表达的变化有关，包括 mga、sagA、csrRS 和 fasBCA。结果表明，Rgg 影响多个调控网络的表达，以共同调控化脓性链球菌中毒力因子的表达。
The human pathogen Streptococcus pyogenes secretes many proteins to the cell wall and extracellular environment that contribute to virulence. Rgg regulates the expression of several exoproteins including a cysteine protease (SPE B), a nuclease (MF-1), a putative nuclease (MF-3), and autolysin. The functional heterogeneity of Rgg-regulated exoproteins and the lack of a conserved regulatory motif in the promoter regions of the genes suggested that Rgg interacts with additional regulatory networks to influence gene expression. DNA microarrays were used to test this hypothesis by comparing genomewide transcript profiles of S. pyogenes NZ131 and isogenic derivative NZ131 rgg during the exponential phase of growth. Transcripts of known and putative virulence-associated genes were more abundant in the rgg mutant, including emm, scpA, orfX, scl1, hasAB, slo, sagA, ska, speH, grab, mac, mf-1, and mf-3. Increased transcription of emm, sepA, and orfX in the rgg mutant was associated with increased production of the corresponding proteins. Differences in the expression of virulence-associated genes were associated with changes in the expression of several regulatory genes, including mga, sagA, csrRS, and fasBCA. The results show that Rgg influences the expression of multiple regulatory networks to coregulate virulence factor expression in S. pyogenes.