Lsh, a SNF2 family member, is required for normal murine development
Lsh, a SNF2 family member, is required for normal murine development
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DOI：
10.1016/s0304-4165(01)00129-5
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发表时间：
2001-05-03
影响因子：
3
通讯作者：
Muegge, K
中科院分区：
文献类型：
--
作者：
Geiman, TM;Tessarollo, L;Muegge, K
Lsh是染色质重塑剂SNF 2家族的成员，可调节复制、修复和转录等多种生物过程。虽然Lsh的表达在成年动物中具有高度的组织特异性，但在胚胎发生过程中，Lsh mRNA在多个组织中均可检测到。为了确定小鼠发育过程中Lsh的生理作用，并评估其在成年小鼠中的独特功能，我们在小鼠胚胎干细胞中使用同源重组进行了Lsh基因的靶向缺失。Lsh-/-胚胎在植入后和胚胎发生期间以预期的孟德尔频率发生。然而，Lsh-/-小鼠在出生后几小时内死亡。此外，新生小鼠的体重比同窝小鼠低22%，并显示肾脏病变。因此，Lsh是SNF 2家族的非冗余成员，对正常小鼠发育和存活至关重要。(C)2001 Elsevier Science B. V.保留所有权利。
Lsh is a member of the SNF2 family of chromatin remodelers, that regulate diverse biological processes such as replication, repair and transcription. Although expression of Lsh is highly tissue specific in adult animals, Lsh mRNA is detectable in multiple tissues during embryogenesis. In order to determine the physiologic role of Lsh during murine development and to assess its unique function in adult mice, we performed targeted deletion of the Lsh gene using homologous recombination in murine embryonic stem cells. Lsh-/- embryos occurred with the expected Mendelian frequency after implantation and during embryogenesis. However, Lsh-/- mice died within a few hours after birth. Furthermore, newborn mice were 22% lower in weight in comparison with their littermates and showed renal lesions. Thus Lsh is a non-redundant member of the SNF2 family and is essential for normal murine development and survival. (C) 2001 Elsevier Science B.V. All rights reserved.