Gastrodia elata modulated activator protein 1 via c-Jun N-terminal kinase signaling pathway in kainic acid-induced epilepsy in rats
Gastrodia elata modulated activator protein 1 via c-Jun N-terminal kinase signaling pathway in kainic acid-induced epilepsy in rats
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DOI：
10.1016/j.jep.2006.07.024
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发表时间：
2007-01-19
影响因子：
5.4
通讯作者：
Ho, Tin-Yun
中科院分区：
文献类型：
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作者：
Hsieh, Ching-Liang;Lin, Jyh-Jian;Ho, Tin-Yun
天麻（兰科）是一种中草药。我们之前的研究表明，天麻能够减少kainic酸（KA）处理大鼠的癫痫发作、氧自由基、小胶质细胞激活和凋亡。激活蛋白1 （Activator protein 1, AP-1）参与调节神经元的可塑性和凋亡。因此，本研究旨在探讨AP-1在天麻抗癫痫作用中的作用。Sprague-Dawley大鼠腹腔注射KA前1周、后2周分别口服天麻（0.5、1.0 g/kg）或丙戊酸（VA, 250 mg/kg）。通过测定c-Jun和c-Fos蛋白来测定AP-1蛋白水平，通过Western blotting测定大鼠脑额叶皮质和海马细胞外信号调节激酶p38和c-Jun n-末端激酶（JNKs）的磷酸化来测定丝裂原活化蛋白（MAP）激酶的激活。上述结果表明，天麻或VA预处理激活了JNK信号通路和c-Jun的表达，而天麻或VA预处理抑制了KA诱导的JNK信号通路和c-Jun的表达。上述结果提示天麻通过JNK信号通路调控ka诱导癫痫AP-1的表达。2006爱思唯尔爱尔兰有限公司版权所有。
Gastrodia elata (Orchidaceae) is a Chinese herb. Our previous study showed that Gastrodia elata is able to reduce epileptic seizures, oxygen free radicals, microglia activation, and apoptosis in kainic acid (KA)-treated rats. Activator protein 1 (AP-1) is involved in modulating the neuronal plasticity and apoptosis. Therefore, the aim of this study was to investigate the role of AP-1 in antiepileptic effect of Gastrodia elata. Gastrodia elata (0.5, 1.0 g/kg) or valproic acid (VA, 250 mg/kg) was administered orally in Sprague-Dawley rats for 1 week before and 2 weeks after intraperitoneal injection of KA. Protein levels of AP-1 were determined by measuring c-Jun and c-Fos proteins, and the mitogen-activated protein (MAP) kinases activations were determined by measuring the phosphorylations of extracellular signal-regulated kinases, p38, and c-Jun N-terminal kinases (JNKs) in the frontal cortex and the hippocampus of rat brain using Western blotting. These results indicated that pre-treatment with Gastrodia elata or VA activated JNK signal pathway and c-Jun expression, while post-treatment with Gastrodia elata or VA suppressed both the JNK signaling pathway and the c-Jun expression induced by KA. These findings suggested that Gastrodia elata regulated the AP-1 expression via the JNK signaling pathway in KA-induced epilepsy. (c) 2006 Elsevier Ireland Ltd. All rights reserved.