Au@Ag Heterogeneous Nanorods as Nanozyme Interfaces with Peroxidase-Like Activity and Their Application for One-Pot Analysis of Glucose at Nearly Neutral pH
Au@Ag Heterogeneous Nanorods as Nanozyme Interfaces with Peroxidase-Like Activity and Their Application for One-Pot Analysis of Glucose at Nearly Neutral pH
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Au@Ag 异质纳米棒作为具有过氧化物酶样活性的纳米酶界面及其在近中性 pH 条件下一锅分析葡萄糖的应用
DOI：
10.1021/acsami.5b03591
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发表时间：
2015-07-08
影响因子：
9.5
通讯作者：
Liu, Aihua
中科院分区：
文献类型：
--
作者：
Han, Lei;Li, Cuncheng;Liu, Aihua
作为天然过氧化物酶的替代品，大多数基于纳米材料的酶模拟物（纳米酶）具有独特的性质，如高稳定性，低成本，大表面积和高催化活性。然而，它们通常在酸性条件下工作，从而阻碍了它们的真实的应用。本工作通过调节双金属材料的纳米结构、组成和表面性质，制备了带正电的聚（二烯丙基二甲基铵）稳定的Au@.Ag异质纳米棒。(NRs)作为协同过氧化物酶样界面，其在宽pH范围内（pH 4.0-6.5）显示出高活性，使用2，2 '-连氮基-双（3-乙基苯并噻唑啉-6-磺酸）二铵盐（ABTS）作为显色底物。在pH 6.5时，Au@Ag异质NR的过氧化物酶样活性在20-40 ℃内是稳定的和最佳的。此外，Au@Ag异质NR显示出优异的温度稳定性和长期储存稳定性。基于这些特性，基于Au@Ag非均相核受体催化H2 O2与ABTS的显色反应，提出了在pH 6.5下检测H2 O2的方法，其中氧化的ABTS在414 nm处显示典型的吸收峰。此外，考虑到Au@Ag异质核受体和葡萄糖氧化酶（GOx）具有相似的催化活性最佳pH，利用GOx，Au@Ag异质核受体的偶联催化反应，和ABTS在接近中性pH（pH 6.5）和37 ℃下。该方法操作简单、快速、线性范围宽（0.05-20 mM），可用于真实的样品的分析。Au@Ag异质核受体具有这些独特的性质，有望成为分析化学和环境科学中过氧化物酶的替代物。
As substitutes for natural peroxidases, most nanomaterial-based enzyme mimetics (nanozymes) have unique properties such as high stability, low-cost, large surface area, and high catalytic activity. However, they usually work in acidic conditions and thus impede their real applications. In this work, by modulating the nanostructure, composition, and surface property of the bimetallic materials, the positively charged poly(diallyldimethylammonium)-stabilized Au@.Ag heterogeneous nanorods.(NRs) were developed as synergistic peroxidase-like interfaces, which exhibited high activity over a wide pH range (pH 4.0-6.5) using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt (ABTS) as the chromogenic substrate. At pH 6.5, the peroxidase-like activity for the Au@Ag heterogeneous NRs was stable and optimal within 20-40 degrees C. Moreover, the Au@Ag heterogeneous NRs showed excellent temperature stability and long-term storage stability. Given these characters, the detection of H2O2 at pH 6.5 was proposed on the basis of the Au@Ag heterogeneous NRs catalyzing the colorimetric reaction of H2O2 and ABTS, where the oxidized ABTS showed a typical absorption peak at 414 nm. The absorbance at 414 nrn was linear with H2O2 concentration from 0.01 to 10 mM. Further, considering that Au@Ag heterogeneous NRs and glucose wddase (GOx) have similar optimal pH for catalytic activities, a novel one-pot method for the detection of glucose was developed by the coupled catalytic reaction using GOx, Au@Ag heterogeneous NRs, and ABTS at nearly neutral pH (pH 6.5) and 37 degrees C. This proposed method had simple and rapid processes, wide linear range (0.05-20 mM), and reliability for the successful analysis of real samples. On the basis of these attractive and unique characteristics, Au@Ag heterogeneous NRs can become promising substitutes for peroxidase in analytical chemistry and environmental science.