Heparin dopant increases the electrical stability, cell adhesion, and growth of conducting polypyrrole/poly(L,L-lactide) composites
Heparin dopant increases the electrical stability, cell adhesion, and growth of conducting polypyrrole/poly(L,L-lactide) composites
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DOI：
10.1002/jbm.a.31735
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发表时间：
2008-11-01
影响因子：
4.9
通讯作者：
Zhang, Ze
中科院分区：
文献类型：
--
作者：
Meng, Shiyun;Rouabhia, Mahmoud;Zhang, Ze
聚吡咯（PPy）是一种在组织工程和生物电应用中具有良好应用前景的导电高分子材料。然而，其导电性在水性条件下容易劣化。细胞对PPy的粘附也相对较差。肝素（HE）是一种多聚苯胺，也是细胞膜和细胞外基质中重要的糖胺聚糖，因此，本研究的目的是利用肝素作为掺杂剂，同时提高PPy的电稳定性和细胞粘附性。以芬顿试剂为氧化剂，通过乳液聚合法合成了HE掺杂聚吡咯（PPy）粒子。采用X射线光电子能谱（XPS）、红外光谱和扫描电子显微镜（SEM）对PPy粒子进行了表征。由具有不同量的HE的5%（w）PPy和95%（w）聚（L，L-丙交酯）（PPy/PLLA）制备10（2）至10（3）Ω/导电生物可降解膜正方形。天青A染色用于定量暴露在PPy颗粒和PPy/PLLA膜表面上的HE。DAPI染色显示HE在膜上的分布。结果表明，HE以反离子的形式存在于PPy粒子表面。在高HE含量下观察到PPy制剂的独特的“细丝”样形态。PPy/PLLA膜的电稳定性在37 ℃的盐水中测试500小时。用人皮肤成纤维细胞检测细胞粘附能力。含有HE掺杂的PPy颗粒的导电膜记录显着增加的电稳定性，细胞粘附和生长。电更稳定和细胞粘附性导电的生物可降解膜可以充当用于各种生物医学应用的平台。(C)2008 Wiley Periodicals，Inc.
Polypyrrole (PPy) is a promising conductive polymer for tissue engineering and bioelectrical applications. However, its electrical conductivity deteriorates easily in aqueous conditions. Cell adhesion to PPy is also relatively poor. The goal of this study was to simultaneously improve the electrical stability of and cell adhesion to PPy by using heparin (HE) as dopant, for HE is both a polyantion and an important glycosaminoglycan in cell membranes and extracellular matrix. PPy particles doped with HE were synthesized through emulsion polymerization using Fenton's reagent as an oxidant. X-ray photoelectron spectroscopy (XPS), infrared and scanning electron microscopy (SEM) were used to investigate the PPy particles. of 10(2) to 10(3) Omega/ Conductive biodegradable membranes square were prepared from 5% (w) PPy with various amounts of HE and 95%, (w) poly(L,L-lactide) (PPy/PLLA). Azure A staining vas employed to quantify the HE exposed on the surface of the PPy particles and PPy/PLLA membranes. The distribution of HE on membranes was demonstrated by DAPI staining. Results showed that HE was incorporated into the PPy particles as counterions and presented on particle Surface. A unique "filament"-like morphology of the PPy preparation was observed at high-HE content. The electrical stability of the PPy/PLLA membranes was tested in saline at 37 degrees C for 500 h. Human skin fibroblasts were used to test the cell adhesion capacity. The conductive membranes containing HE-doped PPy particles recorded significantly increased electrical stability, cell adhesion, and growth. The electrically more stable and cell adhesive conductive biodegradable membrane may act as a platform for various biomedical applications. (C) 2008 Wiley Periodicals, Inc.