Extent of mosaicism in experimentally produced diploid/triploid chimeric trout
Extent of mosaicism in experimentally produced diploid/triploid chimeric trout
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实验生产的二倍体/三倍体嵌合鳟鱼的嵌合程度
DOI：
10.1002/jez.1402660107
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
J. Cloud
中科院分区：
文献类型：
--
作者：
E. Nilsson;J. Cloud
通过将分离的卵裂球注射到完整的受体囊胚中，已经产生了嵌合虹鲑鱼胚胎。为了使嵌合鱼在发育研究中发挥作用，有必要确定所产生的嵌合体的各种器官或组织是否有同样的可能性来自来自捐赠者胚胎的注入细胞。这项调查的目的是确定血液、肝脏和脑组织中来自注射细胞的虹鲑嵌合体细胞的比例。将从正常二倍体囊胚中分离出的卵裂球显微注射到年龄匹配的三倍体受体胚胎中，处理后的胚胎生长到孵化后4周。取血、肝、脑组织标本分散，用碘化丙啶标记细胞核，流式细胞仪分析二倍体和三倍体细胞核的比例。在得到的嵌合体鲑鱼中，二倍体细胞的比例各不相同。各样本二倍体细胞比例的方差分析表明，组织类型对二倍体细胞比例的影响不显著。这些结果支持这样的假设，即供体胚胎的分离细胞并不比另一种组织或器官类型更有可能定植于嵌合体的一种组织或器官类型。©1993 Wiley-Liss，Inc.
Chimeric rainbow trout embryos have been produced by injecting isolated blastomeres into intact recipient blastulae. In order for chimeric fish to be useful in studies of development, it is necessary to determine whether the various organs or tissues of the resultant chimera have an equal probability of arising from the injected cells derived from donor embryos. The objective of this investigation is to determine the proportion of cells in the blood, liver, and brain tissues of rainbow trout chimeras that are derived from the injected cells. Blastomeres isolated from normal diploid blastulae were microinjected into age-matched triploid recipient embryos, and the treated embryos grown until 4 weeks post hatch. Samples of blood, liver, and brain tissues were dispersed; the cell nuclei were labeled with propidium iodide, and the samples subjected to flow cytometric analysis to determine the proportions of diploid and triploid nuclei. The proportion of cells that were diploid varied among the resultant chimeric trout. Analysis of variance on the proportion of diploid cells in each sample indicated that there was no significant effect of tissue type. These results support the hypothesis that the isolated cells of the donor embryo are no more likely to colonize one tissue or organ type of a chimera than another. © 1993 Wiley-Liss, Inc.