Altered cervicovaginal microbiota in premenopausal ovarian cancer patients
Altered cervicovaginal microbiota in premenopausal ovarian cancer patients
复制标题
绝经前卵巢癌患者宫颈阴道微生物群的改变
DOI：
10.1016/j.gene.2021.146083
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发表时间：
2022
期刊：
影响因子：
3.5
通讯作者：
Sutani Takashi
中科院分区：
文献类型：
--
作者：
Morikawa Asuka;Kawabata Ayako;Shirahige Katsuhiko;Akiyama Tetsu;Okamoto Aikou;Sutani Takashi
全世界每年有近30万女性患者被诊断患有卵巢癌，并且这一数字呈上升趋势。然而，卵巢癌引起的特征性症状很少，早期诊断仍然具有挑战性，并且尚未建立有效的筛查方法。在这里，我们在日本进行了一项病例对照研究，使用16S rRNA扩增子测序分析宫颈阴道微生物组与卵巢癌之间的关联。从宫颈涂片样本中提取的DNA分析显示，在绝经前和绝经后健康对照组中，乳酸杆菌占优势，乳酸杆菌缺乏，细菌群落高度多样化，与以前报道的阴道微生物群相同。我们发现，卵巢癌患者的宫颈阴道微生物群，无论其绝经状态如何，通常是一个多样化的社区，与绝经后年龄的健康受试者相似。不同的微生物群与上皮性卵巢癌的主要组织类型相关，包括浆液性卵巢癌和卵巢透明细胞癌。本研究暗示了宫颈阴道微生物生物标志物在绝经前妇女中筛查卵巢癌的潜力。
Nearly three hundred thousand female patients are diagnosed with ovarian cancer in the world annually, and this number shows an increasing trend. However, characteristic symptoms caused by ovarian cancer are so few that early diagnosis remains challenging, and an effective screening method has not yet been established. Here, we conducted a case-control study in Japan to analyze the association between cervicovaginal microbiome and ovarian cancer, using 16S rRNA amplicon sequencing. Analysis of DNA extracted from cervical smear samples revealedLactobacillus-dominant andLactobacillus-deficient, highly-diversified bacterial communities in premenopausal and postmenopausal healthy controls, respectively, as reported for vaginal microbiota previously. We found that cervicovaginal microbiota in ovarian cancer patients, regardless of their menopausal status, were frequently a diversified community and similar to those in healthy subjects at postmenopausal ages. The diverse microbiota was associated with the major histotypes of epithelial ovarian cancer, including serous ovarian cancer and ovarian clear cell cancer. The present study implies the potential of a cervicovaginal microbiome biomarker in screening ovarian cancer in premenopausal women.