VARIABILITY OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 GROUP-O STRAINS ISOLATED FROM CAMEROONIAN PATIENTS LIVING IN FRANCE
VARIABILITY OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 GROUP-O STRAINS ISOLATED FROM CAMEROONIAN PATIENTS LIVING IN FRANCE
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DOI：
10.1128/jvi.69.9.5640-5649.1995
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发表时间：
1995-09-01
影响因子：
5.4
通讯作者：
SARAGOSTI, S
中科院分区：
文献类型：
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作者：
LOUSSERTAJAKA, I;CHAIX, ML;SARAGOSTI, S
人类免疫缺陷病毒1型（HIV-1）编码p24（Gag）和Env C2 V3区的核苷酸序列从7名患者中获得，这些患者是根据HIV酶联免疫吸附试验检测试剂盒子集上的矛盾血清阴性性和非典型Western印迹（免疫印迹）反应性选择的。序列分析显示，所有这些毒株与最近描述的O组（HIV-1异常值）喀麦隆HN分离株的关系比与M组（HIV-1主要）的关系更密切。所有七名患者都有爱沙尼亚血统，但在采集血样时居住在法国。迄今为止，对大量M组菌株的表征揭示了八种不同的遗传亚型（A至H）。现有O组分离株序列之间的遗传距离与M亚型间序列比较中观察到的遗传距离基本相当，表明O组病毒在遗传上非常多样化。对这七种新病毒株的序列分析，结合三种先前表征的O组病毒株，发现在10名患者的病毒序列中几乎没有可辨别的系统发育：聚类模式。O组序列之间的多样性水平表明，它们可能具有与M组序列相当（或更大）的年龄，尽管由于未知原因，后一组序列首先分散，并且是大流行中的主要谱系。
Human immunodeficiency virus type 1 (HIV-1) nucleotide sequences encoding p24(Gag) and the Env C2V3 region were obtained from seven patients,who were selected on the basis of having paradoxical seronegativity on a subset of HIV enzyme linked immunosorbent assay detection kits and having atypical Western blot (immunoblot) reactivity. Sequence analyses showed that all of these strains were more closely related to the recently described Cameroonian HN isolates of group O (HIV-1 outlier) than to group M (HIV-1 major). All seven patients had Cameroonian origins but were living in France at the time the blood samples were taken. Characterization of a large number of group M strains has to date revealed eight distinct genetic subtypes (A to H). Genetic distances between sequences from available group O isolates were generally comparable to those observed in M intersubtype sequence comparisons, showing that the group O viruses are genetically very diverse. Analysis of sequences from these seven new viral strains, combined with the three previously characterized group O strains, revealed few discernable phylogenetic: clustering patterns among the 10 patients' viral sequences. The level of diversity among group O sequences suggests that they may have a comparable (or greater) age than the M group sequences, although for unknown reasons, the latter group dispersed first and is the dominant lineage in the pandemic.