Dynamic Modulation of the Gut Microbiota and Metabolome by Bacteriophages in a Mouse Model
Dynamic Modulation of the Gut Microbiota and Metabolome by Bacteriophages in a Mouse Model
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DOI：
10.1016/j.chom.2019.05.001
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发表时间：
2019-06-12
影响因子：
30.3
通讯作者：
Gerber, Georg K.
中科院分区：
文献类型：
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作者：
Hsu, Bryan B.;Gibson, Travis E.;Gerber, Georg K.
人类肠道微生物组由密集定植的微生物组成，包括噬菌体，它们彼此之间以及哺乳动物宿主之间存在动态相互作用。为了解决噬菌体如何影响肠道细菌群落，我们研究了噬菌体对模型微生物组的动态影响。知生小鼠被确定的人类肠道共生细菌定植，并受到同源裂解噬菌体的捕食。我们发现噬菌体捕食不仅直接影响易感细菌，而且还通过细菌间相互作用对其他细菌物种产生级联效应。代谢组学分析表明，噬菌体捕食引起的微生物组变化对肠道代谢组有直接影响。我们的工作深入了解了噬菌体作为细菌定植调节剂的生态重要性，并且还表明肠道噬菌体对哺乳动物宿主的潜在影响，及其精确调节微生物组的治疗用途的影响。
The human gut microbiome is comprised of densely colonizing microorganisms including bacteriophages, which are in dynamic interaction with each other and the mammalian host. To address how bacteriophages impact bacterial communities in the gut, we investigated the dynamic effects of phages on a model microbiome. Gnotobiotic mice were colonized with defined human gut commensal bacteria and subjected to predation by cognate lytic phages. We found that phage predation not only directly impacts susceptible bacteria but also leads to cascading effects on other bacterial species via interbacterial interactions. Metabolomic profiling revealed that shifts in the microbiome caused by phage predation have a direct consequence on the gut metabolome. Our work provides insight into the ecological importance of phages as modulators of bacterial colonization, and it additionally suggests the potential impact of gut phages on the mammalian host with implications for their therapeutic use to precisely modulate the microbiome.