Thiolation of maghemite nanoparticles by dimercaptosuccinic acid
Thiolation of maghemite nanoparticles by dimercaptosuccinic acid
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DOI：
10.1006/jcis.1997.5125
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发表时间：
1997-10-15
影响因子：
9.9
通讯作者：
Bee, A
中科院分区：
文献类型：
--
作者：
Fauconnier, N;Pons, JN;Bee, A
磁粒子效应偶联物广泛应用于各种病理细胞的体外分选。粒子与效应剂之间的耦合是通过S-S桥实现的，在耦合之前必须先将粒子硫化。在这项工作中，合成，在水介质中，由二巯基琥珀酸硫代磁赤铁矿纳米粒子进行了描述。含硫醇配体的表面络合引起氧化物的还原性溶解，并导致由配体氧化产生的多二硫化物的吸附。吸附和氧化还原反应与培养基的组成密切相关，在不同pH下，添加不同浓度的配体，同时测定了吸附配体的量和释放到培养基中的铁（II）的量。在螯合剂的存在下，粒子的电荷急剧改变；当配体添加量足够时（0.05 mol/mol铁），硫代磁铁矿颗粒基铁磁流体在pH 3 ~ 11范围内稳定，可用于生物医学领域。(C) 1997学术出版社。
Magnetic particle-effector conjugates are widely used in vitro for cell sorting in various pathologies. The coupling between the particles and the effecters being realized through S-S bridges, the particles must first be thiolated before the coupling. In this work, the synthesis, in aqueous medium, of nanoparticles of maghemite thiolated by dimercaptosuccinic acid is described. The superficial complexation by a thiol-containing ligand induces a reductive dissolution of the oxide and leads to the adsorption of polydisulfide species coming from the oxidation of the ligand. Adsorption and redox reactions being strongly correlated to the composition of the medium, the amount of adsorbed ligand and the quantity of iron(II) released into the medium have been simultaneously determined, at various pH, for different concentrations of ligand added. The charge of the particles is drastically modified in the presence of a chelating agent; as a consequence, the colloidal stability is greatly affected and so the flocculation ranges of the complexed particles have been established for different pH. When the quantity of ligand added is sufficient (0.05 mol/mol of iron), the ferrofluid based on thiolated maghemite particles is stable between pH 3 and 11 and can be used for biomedical applications. (C) 1997 Academic Press.