Identification of Taurine-Responsive Genes in Murine Liver Using the Cdo1-Null Mouse Model.
Identification of Taurine-Responsive Genes in Murine Liver Using the Cdo1-Null Mouse Model.
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DOI：
10.1007/978-94-024-1079-2_38
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发表时间：
2017
影响因子：
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通讯作者：
M. Stipanuk;H. Jurkowska;Julie Niewiadomski;Kevin M. Mazor;H. Roman;L. Hirschberger
中科院分区：
文献类型：
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作者：
M. Stipanuk;H. Jurkowska;Julie Niewiadomski;Kevin M. Mazor;H. Roman;L. Hirschberger
The cysteine dioxygenase (Cdo1)-null mouse is unable to synthesize hypotaurine and taurine by the cysteine/cysteine sulfinate pathway and has very low taurine levels in all tissues. The lack of taurine is associated with a lack of taurine conjugation of bile acids, a dramatic increase in the total and unconjugated hepatic bile acid pools, and an increase in betaine and other molecules that serve as organic osmolytes. We used theCdo1-mouse model to determine the effects of taurine deficiency on expression of proteins involved in sulfur amino acid and bile acid metabolism. We identified cysteine sulfinic acid decarboxylase (Csad), betaine:homocysteine methytransferase (Bhmt), cholesterol 7α-hydroxylase (Cyp7a1), and cytochrome P450 3A11 (Cyp3a11) as genes whose hepatic expression is strongly regulated in response to taurine depletion in theCdo1-null mouse. Dietary taurine supplementation ofCdo1-null mice restored hepatic levels of these four proteins and their respective mRNAs to wild-type levels, whereas dietary taurine supplementation had no effect on abundance of these proteins or mRNAs in wild-type mice.
