Behavior of transplanted bone marrow-derived GFP mesenchymal cells in osteochondral defect as a simulation of autologous transplantation
Behavior of transplanted bone marrow-derived GFP mesenchymal cells in osteochondral defect as a simulation of autologous transplantation
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DOI：
10.1369/jhc.4a6280.2005
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发表时间：
2005-02-01
影响因子：
3.2
通讯作者：
Kubo, T
中科院分区：
文献类型：
--
作者：
Oshima, Y;Watanabe, N;Kubo, T
为了阐明自体移植的间充质细胞在骨软骨缺损中的行为，我们使用绿色荧光蛋白（GFP）转基因大鼠跟踪移植细胞，其中所有细胞作为移植供体在其细胞质和细胞核中表达GFP信号。骨髓来源的间充质细胞，其中包含间充质干细胞（MSC），从转基因大鼠获得。然后，将悬滴培养法培养的致密间充质细胞块移植到野生型大鼠骨软骨缺损处，并用纤维蛋白胶固定。术后24周，用透明样软骨和软骨下骨修复缺损。在再生组织中观察到GFP阳性细胞，表明移植的间充质来源的细胞，持续24周，尽管GFP阳性细胞的数量随时间减少。由于GFP不会引起免疫排斥反应，并且不需要化学物质进行可视化，因此转基因大鼠和野生型大鼠之间的移植可以被视为自体移植的模拟，并且移植细胞的存活性能够容易且可靠地跟踪。因此，移植的间充质细胞的行为能够在体内阐明这种策略，其结果可能是必不可少的，在未来的组织工程骨软骨缺损的再生与原始的透明软骨和软骨下骨。
To elucidate the behavior of autologously transplanted mesenchymal cells in osteochondral defects, we followed transplanted cells using green fluorescent protein (GFP) transgenic rats, in which all cells express GFP signals in their cytoplasm and nuclei as transplantation donors. Bone marrow-derived mesenchymal cells, which contain mesenchymal stem cells (MSCs), were obtained from transgenic rats. Then, dense mesenchymal cell masses created by hanging-drop culture were transplanted and fixed with fibrin glue into osteochondral defects of wild-type rats. At 24 weeks after surgery, the defects were repaired with hyaline-like cartilage and subchondral bone. GFP positive cells, indicating transplanted mesenchymal-derived cells, were observed in the regenerated tissues for 24 weeks although GFP positive cells decreased in number with time. Because GFP causes no immunological rejection and requires no chemicals for visualization, transplantation between transgenic and wild-type rats can be regarded as a simulation of autologous transplantation, and the survivability of transplanted cells are able to be followed easily and reliably. Thus, the behavior of transplanted mesenchymal cells was able to be elucidated in vivo by this strategy, and the results could be essential in future tissue engineering for the regeneration of osteochondral defects with original hyaline cartilage and subchondral bone.