Temporal requirement for hedgehog signaling in ventral telencephalic patterning
Temporal requirement for hedgehog signaling in ventral telencephalic patterning
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DOI：
10.1242/dev.01349
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发表时间：
2004-10-01
期刊：
影响因子：
4.6
通讯作者：
Fishell, G
中科院分区：
文献类型：
--
作者：
Fuccillo, M;Rallu, M;Fishell, G
Hedgehog信号传导是大脑发育的多个方面所必需的，包括生长、背侧和腹侧中线图案的建立以及特定细胞类型如少突胶质细胞和中间神经元的产生。为了更精确地鉴定发育期间hedgehog信号传导何时介导这些事件，我们使用FoxG1（Cre）驱动系介导条件性平滑无效等位基因的去除，在胚胎发育的第9天指导脑内hedgehog信号传导的去除。我们观察到腹侧端脑图案的损失，这似乎是由于最初缺乏规范的这些结构，而不是由增殖或细胞死亡的变化。在这些小鼠中去除smoothened的另一个结果是几乎不存在少突胶质细胞和中间神经元。令人惊讶的是，背中线似乎是正常的模式，在这些突变体。与以前的分析一起，目前的结果表明，刺猬信号在E9.0和E12之间的时期是必不可少的腹侧区域的图案和产生的细胞类型，被认为主要是从他们。
Hedgehog signaling is required for multiple aspects of brain development, including growth, the establishment of both dorsal and ventral midline patterning and the generation of specific cell types such as oligodendrocytes and interneurons. To identify more precisely when during development hedgehog signaling mediates these events, we directed the removal of hedgehog signaling within the brain by embryonic day 9 of development, using a FoxG1(Cre) driver line to mediate the removal of a conditional smoothened null allele. We observed a loss of ventral telencephalic patterning that appears to result from an initial lack of specification of these structures rather than by changes in proliferation or cell death. A further consequence of the removal of smoothened in these mice is the near absence of both oligodendrocytes and interneurons. Surprisingly, the dorsal midline appears to be patterned normally in these mutants. Together with previous analyses, the present results demonstrate that hedgehog signaling in the period between E9.0 and E12 is essential for the patterning of ventral regions and the generation of cell types that are thought to largely arise from them.