SMOOTHING + DIFFERENTIATION OF DATA BY SIMPLIFIED LEAST SQUARES PROCEDURES
SMOOTHING + DIFFERENTIATION OF DATA BY SIMPLIFIED LEAST SQUARES PROCEDURES
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DOI：
10.1021/ac60214a047
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发表时间：
1964-01-01
影响因子：
7.4
通讯作者：
GOLAY, MJE
中科院分区：
文献类型：
--
作者：
SAVITZKY, A;GOLAY, MJE
干涉。二氯甲烷或氯仿可溶的羧酸(乙酰水杨酸、水杨酸等)用0.1Ar氢氧化钠从这些相中很容易地提取出抑制4-AAP反应的化合物。对4-AAP试剂有轻微反应的Span类辅料在10%的氯化钠溶液中不溶而被消除，而干扰吐温类辅料则用吐温试剂沉淀(8)。当丙二醇的配方为5.25%时，对观察到的吸光度的贡献约为2%至3%。通过在程序中使用的水相中更有利地分配干扰来最小化这一点。
Interferences. Methylene chloride or chloroform-soluble carboxylic acids (acetylsalicylic acid, salicylic acid, etc.) which inhibited 4-AAP response were readily extracted from these phases using 0.1 Ar sodium hydroxide. Span-type excipients, which gave a slight response to the 4-AAP reagent, were eliminated by virtue of their insol-ubility in 10% aqueous sodium chloride; while interfering Tween-type excipients were precipitated (8) using the Tween reagent. Propylene glycol, when formulated at a level of 5.25%, contributed ca. 2 to 3% to the observed absorbance. This was minimized by a more favorable distribution of the interference within the aqueous phasesused in the pro-cedure.