A Miniaturized Hemoretractometer for Blood Clot Retraction Testing.
A Miniaturized Hemoretractometer for Blood Clot Retraction Testing.
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DOI：
10.1002/smll.201600274
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发表时间：
2016-08
期刊：
影响因子：
--
通讯作者：
Fu J
中科院分区：
文献类型：
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作者：
Li Z;Li X;McCracken B;Shao Y;Ward K;Fu J
凝血是一个关键的止血过程，必须适当调节以维持出血和凝血之间的微妙平衡。凝血障碍可使患者暴露于出血性疾病或血栓性疾病的风险。使用全血的凝血诊断对于评估凝血系统的复杂性和止血的整体测量是非常有希望的。尽管现有的全血凝固测试已经证明了临床价值，但是这些系统具有显著的局限性，这降低了它们用于即时护理应用的潜力。在这项工作中，我们利用最新的进展，在设备小型化使用功能软材料开发一个小型化的血栓收缩力测定设备称为mHemoRetractoMeter（mHRM）。mHRM能够使用微量全血在真实的时间内精确测量动态凝块收缩力。为了进一步证明mHRM的临床实用性，我们使用mHRM进行了系统研究，以检查测定温度、凝血剂处理以及促凝和抗凝药物对全血样本凝块收缩力发展的影响。mHRM的制造成本低，尺寸小，仅消耗少量血液样本，使该技术有望成为未来凝血监测的即时护理工具。开发了一种小型血液牵拉仪，用于使用微量全血来真实的测量动态血栓牵拉力。该装置利用血液样本的界面行为，将其定位在两个独立的柔性梁之间，作为力传感器。证明了用于全血测量的血液折射计的潜在临床应用。
Blood coagulation is a critical hemostatic process that must be properly regulated to maintain a delicate balance between bleeding and clotting. Disorders of blood coagulation can expose patients to the risk of either bleeding disorders or thrombotic diseases. Coagulation diagnostics using whole blood is very promising for assessing the complexity of the coagulation system and for global measurements of hemostasis. Despite the clinic values that existing whole blood coagulation tests have demonstrated, these systems have significant limitations that diminish their potential for point-of-care applications. In this work, we leverage recent advancements in device miniaturization using functional soft materials to develop a miniaturized clot retraction force assay device termed mHemoRetractoMeter (mHRM). The mHRM is capable of precise measurements of dynamic clot retraction forces in real time using minute amounts of whole blood. To further demonstrate the clinical utility of the mHRM, we conduct systematic studies using the mHRM to examine the effects of assay temperature, treatments of clotting agents, and pro- and anti-coagulant drugs on clot retraction force developments of whole blood samples. The mHRM's low fabrication cost, small size, and consumption of only minute amounts of blood samples make the technology promising as a point-of-care tool for future coagulation monitoring. A miniaturized hemoretractometer is developed to measure dynamic clot retraction forces in real time using minute amounts of whole blood. This device takes advantage of interfacial behavior of blood samples for their localization between two free-standing flexible beams serving as force sensors. Potential clinical applications of the hemoretractometer for whole-blood measurements are demonstrated.