Dynamic contrast-enhanced photoacoustic imaging using photothermal stimuli-responsive composite nanomodulators.
Dynamic contrast-enhanced photoacoustic imaging using photothermal stimuli-responsive composite nanomodulators.
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DOI：
10.1038/ncomms15782
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发表时间：
2017-06-08
影响因子：
16.6
通讯作者：
Emelianov S
中科院分区：
文献类型：
--
作者：
Chen YS;Yoon SJ;Frey W;Dockery M;Emelianov S
分子光声成像在医学应用中显示出巨大的潜力，其灵敏度通常在皮至微摩尔范围内，依赖于外源性成像剂。然而，组织可产生强背景信号，其掩蔽来自成像剂的信号，导致数量级的灵敏度降低。因此，通常需要精细的光谱扫描以在光谱上解混不想要的背景信号。在这里，我们展示了一种新的单波长光声动态对比增强成像技术，通过采用刺激响应造影剂。我们的技术可以消除固有的背景噪声，没有显着的硬件或计算资源。我们表明，这种新的造影剂可以产生比浓度匹配的无机纳米粒子对应物强30倍的光声信号。通过用外部近红外光刺激动态调制来自造影剂的信号，我们可以进一步抑制背景信号，导致体内成像对比度额外增加超过五倍。光声成像技术是一种用于临床疾病诊断的使能技术。在这里，Chen等人报道了一种成像造影剂-笼在水凝胶中的等离子体纳米颗粒，其经受取决于温度的可逆体积变化，其表现出可调谐的光声信号。
Molecular photoacoustic imaging has shown great potential in medical applications; its sensitivity is normally in pico-to-micro-molar range, dependent on exogenous imaging agents. However, tissue can produce strong background signals, which mask the signals from the imaging agents, resulting in orders of magnitude sensitivity reduction. As such, an elaborate spectral scan is often required to spectrally un-mix the unwanted background signals. Here we show a new single-wavelength photoacoustic dynamic contrast-enhanced imaging technique by employing a stimuli-responsive contrast agent. Our technique can eliminate intrinsic background noises without significant hardware or computational resources. We show that this new contrast agent can generate up to 30 times stronger photoacoustic signals than the concentration-matched inorganic nanoparticle counterparts. By dynamically modulating signals from the contrast agents with an external near-infrared optical stimulus, we can further suppress the background signals leading to an additional increase of more than five-fold in imaging contrast in vivo. Photoacoustic imaging becomes an enabling technology that is designed for clinic diagnosis of disease. Here, Chen et al. report an imaging contrast agent—plasmonic nanoparticles caged in hydrogel subject to reversible volume change depending on temperature, which exhibits tunable photoacoustic signal.