Retinas of the Diurnal Rodent Arvicanthis ansorgei Are Highly Resistant to Experimentally Induced Stress and Degeneration
Retinas of the Diurnal Rodent Arvicanthis ansorgei Are Highly Resistant to Experimentally Induced Stress and Degeneration
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DOI：
10.1167/iovs.11-8162
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发表时间：
2011-11-01
影响因子：
4.4
通讯作者：
Hicks, David
中科院分区：
文献类型：
--
作者：
Boudard, Domitille L.;Acar, Niyazi;Hicks, David
目的.环境引起的压力在动物和人类的视网膜变性和失明中起着重要作用。在这些压力来源中，光毒性已得到充分研究，并已显示在一些物种中导致光感受器特异性丧失。然而，绝大多数研究都是在夜间活动的白化病杆状优势大鼠和小鼠品系中进行的，这些发现与人类病理学和视锥细胞丢失的相关性值得商榷。作者研究了白天活动的鼠类啮齿动物安氏阿氏Arvicanthis ansorgei的视网膜损伤脆弱性，这是一种具有大量视锥的有色物种。作者使用已建立的协议将动物暴露于广泛的光照条件（可变强度，持续时间，光谱，先前的光照历史和暴露时间）和注射N-甲基-N-亚硝基脲（MNU）;据报道，每个程序都会产生快速和完全的感光细胞特异性损伤。然后对动物进行视网膜电描记术以记录视杆细胞和视锥细胞的功能，随后对动物实施安乐死，并用于视网膜结构的免疫组织化学分析和游离脂肪酸的定量。这些标准方案对A没有产生可检测的有害影响。安氏视网膜表型、功能或结构。A.视网膜部分损伤;安氏病由非常强的蓝光或高剂量的MNU诱导。这种抗性不是由于A.安氏杆菌和敏感品系的小鼠和大鼠。该物种的视网膜对光线和毒素（如MNU）的损害具有极高的抵抗力。（Invest Ophthalmol维斯科学。2011;52：8686-8700）DOI：10.1167/iovs.11-8162
PURPOSE. Environmentally induced stress plays a significant role in retinal degeneration and blindness both in animals and in humans. Among such sources of stress, phototoxicity is well studied and has been shown to lead to photoreceptor-specific loss in a number of species. However, the vast majority of studies have been conducted in nocturnal, albino rod-dominant rat and mouse strains, and the pertinence of such findings to human pathology and cone loss is debatable. The authors examined retinal vulnerability to damage in the diurnal murid rodent Arvicanthis ansorgei, a pigmented species with a large number of cones.METHODS. The authors used established protocols for exposing animals to a wide range of lighting conditions (variable intensity, duration, spectrum, previous light history, and time of exposure) and injecting N-methyl-N-nitrosourea (MNU); each procedure is reported to produce rapid and complete photoreceptor-specific damage. Animals then underwent electroretinography to record rod and cone function and were subsequently euthanized and used for immunohistochemical analysis of retinal structure and quantification of free fatty acids.RESULTS. These standard regimens produced no detectable detrimental effects on A. ansorgei retinal phenotype, function, or structure. Partial retinal damage in A. ansorgei was induced by very intense blue light or elevated doses of MNU. This resistance was not attributable to differences in lipid composition (specifically, docosahexaenoic acid) between A. ansorgei and susceptible strains of mice and rats.CONCLUSIONS. The retina of this species exhibits exceptionally high resistance to damage from light and toxins such as MNU. (Invest Ophthalmol Vis Sci. 2011;52:8686-8700) DOI: 10.1167/iovs.11-8162