Arabidopsis hapless mutations define essential gametophytic functions
Arabidopsis hapless mutations define essential gametophytic functions
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DOI：
10.1534/genetics.104.029447
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发表时间：
2004-10-01
期刊：
影响因子：
3.3
通讯作者：
Preuss, D
中科院分区：
文献类型：
--
作者：
Johnson, MA;von Besser, K;Preuss, D
在开花植物中，卵在单倍体胚囊(雌配子字节)内发育，该胚囊被包裹在雌蕊内。单倍体花粉粒(雄配子体)将细胞质内携带两个精子的花粉管延伸到胚囊。这一壮举需要通过柱头、花柱和子房的细胞、柱头和子房的细胞之间和表面快速、精确地引导和高度极化的生长。花粉管的迁移依赖于二倍体雌性细胞发出的一系列远程信号，以及胚囊发出的引导花粉管生长最后阶段的短程引诱剂。我们在拟南芥中开发了一种遗传屏幕，它用插入元件上携带的细胞自主标记来标记突变的花粉。我们发现了32个单倍体破坏性(倒霉)突变，这些突变定义了花粉粒发育、柱头和花柱中的花粉管生长、或子房中的花粉管生长和引导所需的基因。我们还鉴定了11个hap突变体插入元件两侧的基因组DNA，并表明hap1破坏了AtMago基因，AtMago基因的同源基因对果蝇细胞的极性很重要。
In flowering plants, the egg develops within a haploid embryo sac (female gametopbyte) that is encased within the pistil. The haploid pollen grain (male gametophyte) extends a pollen tube that carries two sperm cells within its cytoplasm to the embryo sac. This feat requires rapid, precisely guided, and highly polarized growth through, between, and on the surface of the cells of the stigma, style, and ovary. Pollen tube migration depends on a series of long-range signals from diploid female cells as well as a short-range attractant emitted by the embryo sac that guides the final stage of tube growth. We developed a genetic screen in Arabidopsis thaliana that tags mutant pollen with a cell-autonomous marker carried on an insertion element. We found 32 haploid-disrupting (hapless) mutations that define genes required for pollen grain development, pollen tube growth in the stigma and style, or pollen tube growth and guidance in the ovary. We also identified genomic DNA flanking the insertion element for eleven hap mutants and showed that hap1 disrupts AtMago, a gene whose ortholog is important for Drosophila cell polarity.