Elevation of conjugation capacity in isolated hepatocytes from BHA-treated mice.
Elevation of conjugation capacity in isolated hepatocytes from BHA-treated mice.
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BHA 处理小鼠分离肝细胞的结合能力提高。
DOI：
10.1016/0006-2952(82)90496-8
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发表时间：
1982
影响因子：
5.8
通讯作者：
Jernström,B
中科院分区：
文献类型：
--
作者：
Moldéus,P;Dock,L;Cha,YN;Berggren,M;Jernström,B
使用骆驼蓬酚和对乙酰氨基酚作为底物，通过BHA喂养的缀合反应的升高及其对该抗氧化剂的保护作用的意义已经用从小鼠获得的肝细胞进行了研究。使用两种底物，观察到葡萄糖醛酸化几乎升高5倍。然而，硫酸盐结合率没有变化。对乙酰氨基酚的谷胱甘肽结合物形成率也没有增强，即使GSH水平和谷胱甘肽S-转移酶活性都增加了。最后，BHA给药对扑热息痛的肝毒性作用没有保护作用，即使3-甲基胆蒽预处理增加了反应性扑热息痛代谢产物的产生。
Using harmol and paracetamol as the substrates, the elevation of conjugation reactions by BHA feeding and their significance towards the protective effects of this antioxidant has been studied with hepatocytes obtained from mice. With both substrates, an almost five fold elevation of the glucuronidation was observed. However, there was no change in the rate of sulfate conjugation. The rate of glutathione conjugate formation with paracetamol was also not enhanced, even though both the GSH level and glutathioneS-transferase activities were increased. Finally, BHA administration afforded no protective effect against the hepatotoxic effects of paracetamol, even when the production of reactive paracetamol metabolites was increased by 3-methylcholanthrene pretreatment.