Determination of sinigrin and glucoraphanin in Brassica species using a simple extraction method combined with ion-pair HPLC analysis
Determination of sinigrin and glucoraphanin in Brassica species using a simple extraction method combined with ion-pair HPLC analysis
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DOI：
10.1016/s0304-4238(02)00119-x
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发表时间：
2002-12
影响因子：
4.3
通讯作者：
Nuchanart Rangkadilok;M. Nicolas;R. Bennett;R. Premier;D. Eagling;P. Taylor
中科院分区：
文献类型：
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作者：
Nuchanart Rangkadilok;M. Nicolas;R. Bennett;R. Premier;D. Eagling;P. Taylor
使用简单可靠的提取方法测定了甘蓝属植物种子和幼苗中黑芥苷（2-丙烯基芥子油苷）和萝卜硫苷（4-甲基亚磺酰基丁基芥子油苷）浓度的分布：甘蓝、黑芥子、芥菜、白菜和欧洲油菜。使用沸水从种子和幼苗中提取芥子油苷，并通过反相离子对色谱法和紫外检测进行分析。在 B. nigra、B.juncea var. 中发现了高浓度的黑芥苷。 rugosa（芥菜）和 B. oleracea（抱子甘蓝、卷心菜、花椰菜和西兰花）。白菜和甘蓝型油菜中不存在芥子苷，而在甘蓝中的浓度可以忽略不计。意大利（西兰花）。在大多数测试的芸苔属植物中，种子中黑芥子苷的浓度与子叶和下胚轴组织（7 日龄幼苗）中的浓度相似。在甘蓝变种中发现了高浓度的萝卜硫苷。 italica 和中等浓度的 B. oleracea var. capitata（卷心菜），而 B. nigra、B. juncea、B. rapa 和 B. napus 中不存在。西兰花基因型中萝卜硫苷浓度在 44.2 至 274.1μmolg−1DW 之间。 Kalibrini® 是西兰花和中国西兰花（芥兰）之间的新杂交品系，与其亲本品系（西兰花中为 274.1μmolg−1DW，但芥兰中仅为 1.2μmolg−1DW）相比，含有中等浓度的萝卜硫素（145.3μmolg−1DW）。幼苗中的萝卜硫苷浓度显着低于西兰花种子中的浓度，而大多数测试的芸苔属植物幼苗中的黑芥子苷浓度没有显着差异。该研究表明，可供选择和育种的芸苔属物种和基因型之间的黑芥子苷和萝卜硫苷浓度存在广泛的变异性。可以生产新的精英品系，用作保健食品或营养保健品行业的经济作物。
Distribution of sinigrin (2-propenylglucosinolate) and glucoraphanin (4-methylsulphinylbutylglucosinolate) concentration was determined in seeds and seedlings of Brassica species: B. oleracea, B. nigra, B.juncea, B. rapa and B. napus using a simple and reliable extraction method. Glucosinolates were extracted from seeds and seedlings using boiling water and were analysed by reverse phase ion-pair chromatography with UV detection. High concentrations of sinigrin were found in B. nigra, B.juncea var. rugosa (mustard greens) and B. oleracea (Brussels sprouts, cabbage, cauliflower, and Chinese broccoli). Sinigrin was absent from B. rapa and B. napus and was present in negligible concentrations in B. oleracea var. italica (broccoli). The concentration of sinigrin in the seed was similar to that in the cotyledon and hypocotyl tissues (7-day-old seedling) in most Brassica species tested. Glucoraphanin was found at high concentrations in B. oleracea var. italica and moderate concentrations in B. oleracea var. capitata (cabbage), whilst was absent in B. nigra, B. juncea, B. rapa and B. napus. Glucoraphanin concentration was between 44.2 and 274.1μmolg−1DW among broccoli genotypes. Kalibrini®, a new hybrid line between broccoli and Chinese broccoli (kailan) contained a moderate concentration of glucoraphanin (145.3μmolg−1DW) compared to its parent lines (274.1μmolg−1DW in broccoli but only 1.2μmolg−1DW in kailan). Glucoraphanin concentration in seedlings was significantly lower than that in the seed of broccoli while there was no significant difference in sinigrin concentration in seedlings of most Brassica species tested. The study has shown the extensive variability in sinigrin and glucoraphanin concentrations among Brassica species and genotypes that is available for selection and breeding. New elite lines can be produced for use as commercial crops in the health food or nutraceutical industries.