Differential expression of sodium channel genes in retinal ganglion cells
Differential expression of sodium channel genes in retinal ganglion cells
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DOI：
10.1016/s0169-328x(97)00187-3
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发表时间：
1997-10-15
期刊：
影响因子：
--
通讯作者：
Waxman, SG
中科院分区：
文献类型：
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作者：
Fjell, J;DibHajj, S;Waxman, SG
视网膜神经节细胞轴突中的动作电位电发生由电压门控钠通道支持，并且河豚毒素（TTX）可耐受的钠电导参与视神经内这些轴突的缺氧损伤。然而，在视网膜神经节细胞中表达的钠通道的亚型尚未确定。在这项研究中，我们使用逆转录-聚合酶链反应（RT-PCR）和限制性内切酶图谱，连同原位杂交，检查转录本钠通道α亚基I，II，III，NaG，Na 6，hNE/PN 1和SNS，和β亚基1和2，在成年大鼠视网膜的表达。RT-PCR产生了I、II、III、Na 6、β（1）和β（2）转录物的高水平扩增。原位杂交证实了所有这些mRNA的存在下，在视网膜神经节细胞的细胞体。因此，视网膜神经节细胞表达多种钠通道mRNA，表明它们部署了几种不同类型的钠通道。(C)1997年Elsevier Science B.V.
Action potential electrogenesis in the axons of retinal ganglion cells is supported by voltage-gated sodium channels, and a tetrodotoxin (TTX)-inhibitable sodium conductance participates in anoxic injury of these axons within the optic nerve. However, the subtypes of sodium channels expressed in retinal ganglion cells have not been identified. In this study, we used reverse transcription-polymerase chain reaction (RT-PCR) and restriction enzyme mapping, together with in situ hybridization, to examine the expression of transcripts for sodium channel alpha-subunits I, II, III, NaG, Na6, hNE/PN1 and SNS, and beta-subunits 1 and 2, in the retina of the adult rat. RT-PCR yielded high levels of amplification of I, II, III, Na6, beta(1) and beta(2) transcripts. In situ hybridization demonstrated the presence of all these mRNAs in the cell bodies of retinal ganglion cells. Retinal ganglion cells thus express multiple sodium channel mRNAs, suggesting that they deploy several different types of sodium channels. (C) 1997 Elsevier Science B.V.