Genetic screens in Saccharomyces cerevisiae identify a role for 40S ribosome recycling factors Tma20 and Tma22 in nonsense-mediated decay.
Genetic screens in Saccharomyces cerevisiae identify a role for 40S ribosome recycling factors Tma20 and Tma22 in nonsense-mediated decay.
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酿酒酵母的遗传筛选确定了 40S 核糖体回收因子 Tma20 和 Tma22 在无义介导的衰变中的作用。
DOI：
10.1093/g3journal/jkad295
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发表时间：
2024
期刊：
影响因子：
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通讯作者：
Green,Rachel
中科院分区：
文献类型：
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作者：
Pacheco,Miguel;D'Orazio,KaroleN;Lessen,LauraN;Veltri,AnthonyJ;Neiman,Zachary;Loll-Krippleber,Raphael;Brown,GrantW;Green,Rachel
无义介导的降解（NMD）是真核生物的一条重要调控途径，也是哺乳动物的一条重要途径。NMD通常由终止于异常远离聚腺苷酸尾的终止密码子的核糖体触发。在这里，我们使用荧光筛选来确定参与NMD inSaccharomycescerevisiae的因子。除了已知的NMD因素，包括整个UPF家族（UPF 1，UPF 2和UPF 3），以及NMD 4和EBS 1，我们确定了已知的因素在终止后再循环和表征其对NMD的贡献。这些在S. Parae-expand的观察结果扩展了哺乳动物中的数据，表明60 S再循环因子ABCE 1对NMD很重要，因为40 S再循环中涉及的因子的扰动也与NMD的丧失相关。
The decay of messenger RNA with a premature termination codon by nonsense-mediated decay (NMD) is an important regulatory pathway for eukaryotes and an essential pathway in mammals. NMD is typically triggered by the ribosome terminating at a stop codon that is aberrantly distant from the poly-A tail. Here, we use a fluorescence screen to identify factors involved in NMD inSaccharomyces cerevisiae. In addition to the known NMD factors, including the entire UPF family (UPF1, UPF2, and UPF3), as well asNMD4andEBS1, we identify factors known to function in posttermination recycling and characterize their contribution to NMD. These observations inS. cerevisiaeexpand on data in mammals indicating that the 60S recycling factor ABCE1 is important for NMD by showing that perturbations in factors implicated in 40S recycling also correlate with a loss of NMD.