Streptococcus mutans binding to solid phase dextran mediated by the glucan-binding protein C.
Streptococcus mutans binding to solid phase dextran mediated by the glucan-binding protein C.
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DOI：
10.1034/j.1399-302x.2002.170408.x
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发表时间：
2002-08
影响因子：
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通讯作者：
Y. Sato;H. Senpuku;K. Okamoto;N. Hanada;H. Kizaki
中科院分区：
文献类型：
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作者：
Y. Sato;H. Senpuku;K. Okamoto;N. Hanada;H. Kizaki
变形链球菌GbpC是一种壁锚定的表面蛋白，其参与葡聚糖依赖性聚集。GbpC表型仅在应激条件下生长的细胞中观察到。为了检测S.变形链球菌的GbpC蛋白，我们用N-乙酰胞壁酶消化该生物体的细胞壁后分离壁组分，并检测来自S.通过用抗GbpC血清进行蛋白质印迹分析来检测变形细胞。有趣的是，S.表现出负葡聚糖（α-1，6葡聚糖）依赖性聚集（DDAG）表型的变形细胞表达该蛋白质，并且可以与固定的葡聚糖结合。
Streptococcus mutans GbpC is a wall-anchored surface protein which is involved in dextran-dependent aggregation. The GbpC phenotype is observed only in cells grown under stress conditions. In order to detect the GbpC protein of S. mutans, we isolated the wall fraction following digestion of the cell wall of this organism by N-acetylmuramidase, and detected the GbpC protein from S. mutans cells by western analysis with anti-GbpC serum. Interestingly, S. mutans cells exhibiting the negative dextran(alpha-1,6 glucan)-dependent aggregation (ddag) phenotype expressed the protein and could bind to immobilized dextran.