Molecular cloning and characterization of DEC2, a new member of basic helix-loop-helix proteins
Molecular cloning and characterization of DEC2, a new member of basic helix-loop-helix proteins
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DOI：
10.1006/bbrc.2000.4133
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发表时间：
2001-01-12
影响因子：
3.1
通讯作者：
Kato, Y
中科院分区：
文献类型：
--
作者：
Fujimoto, K;Shen, M;Kato, Y
DEC 1是一个基本的螺旋-环-螺旋（bHLH）蛋白相关的果蝇毛，分裂和羟乙基淀粉增强剂，并参与控制的增殖和/或软骨细胞，神经元等的分化，我们在这里报告的识别和表征的人，小鼠和大鼠DEC 2，DEC亚家族的一个新成员。DEC 2与DEC 1在bHLH区域和橙子结构域分别具有高（97%）和中等（52%）相似性。然而，德克斯，而不是DEC 1，在C-末端的一半有丙氨酸和甘氨酸丰富的区域。与Hairy、分裂增强子和HES不同，德克斯缺乏与辅阻遏物Groucho相互作用的WRPW基序。DEC 2基因定位于人染色体12 p11.23-p12.1、小鼠染色体6 G2-G3和大鼠染色体4 q43 distal-q4，在这些物种中已经确定了保守的连锁同源性。与DEC 1在许多组织中广泛表达不同，DEC 2显示出更受限制的mRNA表达模式。DEC亚家族蛋白可能在组织发育中起重要作用。(C)北京：科学出版社.
DEC1 is a basic helix-loop-helix (bHLH) protein related to Drosophila Hairy, Enhancer of split and HES, and involved in the control of proliferation and/or differentiation of chondrocytes, neurons, etc. We report here the identification and characterization of human, mouse and rat DEC2, a novel member of the DEC subfamily. DEC2 had high (97%) and moderate (52%) similarities in the bHLH region and the Orange domain with DEC1, respectively. However, DECS, but not DEC1, had alanine and glycine-rich regions in the C-terminal half. Unlike Hairy, Enhancer of split and HES, DECS lacked the WRPW motif for interaction with the corepressor Groucho. The DEC2 gene was mapped to human chromosome 12p11.23-p12.1, mouse chromosome 6 G2-G3 and rat chromosome 4q43 distal-q4, where the conserved linkage homology has been identified among these species. Unlike DEC1, which was broadly expressed in many tissues, DEC2 showed a more restricted pattern of mRNA expression. The DEC subfamily proteins may play an important role in tissue development. (C) 2001 Academic Press.