Cardiorespiratory and metabolic responses to injection of bicuculline into the hypothalamic paraventricular nucleus (PVN) of conscious rats
Cardiorespiratory and metabolic responses to injection of bicuculline into the hypothalamic paraventricular nucleus (PVN) of conscious rats
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DOI：
10.1016/s0006-8993(01)02011-x
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发表时间：
2001-03-23
期刊：
影响因子：
2.9
通讯作者：
Martin, D
中科院分区：
文献类型：
--
作者：
Schlenker, E;Barnes, L;Martin, D
刺激 PVN 会增加平均动脉压 (MAP) 和心率 (HR)。然而，人们对其调节通气的作用知之甚少。我们测试了以下假设：通过显微注射甲硫荷包牡丹碱 (BMI)（一种 γ-氨基丁酸 (GABA)）受体拮抗剂刺激 PVN，可增加清醒大鼠的通气量。还评估了耗氧量，以确定通气反应是否与代谢率增加相关。给雄性 Sprague-Dawley 大鼠安装股骨导管来测量 MAP 和 HR，并将插管植入 PVN 上方 1 毫米处。经过5~7天的恢复、代谢。评估了人工脑脊液（aCSF）和体重指数的通气和心血管反应。向大鼠单侧显微注射 50 nl aCSF（媒介物对照），然后向另一侧注射 50 nl BMI (1 mM)。与 aCSF 相比，BMI 显微注射显着增加了 MAP（145 +/-4 vs. 124 +/-5 mmHg，P
Stimulation of the PVN increases mean arterial pressure (MAP) and heart rate (HR). However, little is known about its role in modulating ventilation. We tested the hypothesis that the stimulation of the PVN by microinjection of bicuculline methiodide (BMI), a gamma -aminobutyric acid (GABA), receptor antagonist, increases ventilation in conscious rats. Oxygen consumption was also evaluated to determine if the ventilatory responses were associated with increases in metabolic rate. Male Sprague-Dawley rats were instrumented with femoral catheters to measure MAP and HR and cannulae were implanted 1 mm above the PVN. After 5 to 7 days of recovery, metabolic. ventilatory, and cardiovascular responses to artificial cerebrospinal fluid (aCSF) and BMI were evaluated. Rats were given a 50 nl unilateral microinjection of aCSF (the vehicle control) followed by 50 nl of BMI (1 mM) into the other side. Microinjection of BMI significantly increased MAP compared to aCSF (145 +/-4 vs. 124 +/-5 mmHg, P