CONCENTRATIONS OF 2-HYDROXYESTROGENS IN HUMAN-SERA MEASURED BY A HETEROLOGOUS IMMUNOASSAY WITH AN I-125-LABELED LIGAND
CONCENTRATIONS OF 2-HYDROXYESTROGENS IN HUMAN-SERA MEASURED BY A HETEROLOGOUS IMMUNOASSAY WITH AN I-125-LABELED LIGAND
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DOI：
10.1530/acta.0.1000154
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发表时间：
1982-01-01
期刊：
影响因子：
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通讯作者：
KUSS, E
中科院分区：
文献类型：
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作者：
BERG, D;THALER, F;KUSS, E
建立了2-羟基雌激素的异源免疫测定，其中针对2-羟基雌激素-17-琥珀酰-牛血清白蛋白产生的抗体用作结合蛋白，而17-O-[N-β- [125 I]碘-咪唑基）乙基羧酰胺基甲基]肟基-2，3-二羟基-1，3，5（10）-雌三烯作为放射性配体。在该系统中竞争结合位点的亲脂性血清组分定义为总2-羟基雌激素。特异性的基本假设得到了用结构相关类固醇和邻苯二酚评价的交叉反应性模式的支持，并证实了竞争反应前血清提取物的额外色谱法几乎没有影响（如果有）的事实。灵敏度：2.8 ±。1 pg/管;准确度：Y = 0.91x + 2.2; r = 0.989;精密度：批内5.8%;批间6.5%。以下浓度（±.标准偏差）。青年男子：29岁+-。5 pg/ml（n = 11）;女性卵泡期：32 . ±. 8 pg/ml（n = 25）;黄体期：53 ±。13 pg/ml（n = 23）;绝经后妇女：13 . ±. 4 pg/ml（n = 10）;第11 - 20周孕妇：70 ±。16 mg/ml（n = 64）;第36 - 40周：240 . ±. 23 pg/ml（n = 40）;新生儿脐带血：604 ±。43 pg/ml（n = 48）。
A heterologous immunoassay for 2-hydroxyestrogens was established in which antibodies raised against 2-hydroxyestradiol-17-succinyl-bovine serum albumin serve as binding protein and 17-O-[N-.beta.-[125I]iodo-imidazolyl)ethylcarboxyamidomethyl]oximino-2,3-dihydroxy-1,3,5(10)-estratriene as radioligand. Lipophilic serum components competing for binding sites in this system were defined as total 2-hydroxyestrogens. The underlying assumption of specificity was supported by the pattern of cross-reactivity evaluated with structural related steroids and o-diphenols and byl the fact, that an additional chromatography of the serum extracts preceding the competing reaction had little if any effect. Sensitivity: 2.8 .+-. 1 pg/tube; accuracy: Y = 0.91x + 2.2; r = 0.989; precision: 5.8% intra-assay; 6.5% inter-assay. The following concentrations (.+-. standard deviation) were found in the sera of healthy subjects. Young men: 29 .+-. 5 pg/ml (n = 11); women follicular phase: 32 .+-. 8 pg/ml (n = 25); luteal phase: 53 .+-. 13 pg/ml (n = 23); postmenopausal women: 13 .+-. 4 pg/ml (n = 10); pregnant women 11th-20th week: 70 .+-. 16 mg/ml (n = 64); 36th-40th week: 240 .+-. 23 pg/ml (n = 40); newborn cord blood: 604 .+-. 43 pg/ml (n = 48).