Molecular Genetic Analysis of a Human Neuropeptide Y Receptor
Molecular Genetic Analysis of a Human Neuropeptide Y Receptor
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人类神经肽 Y 受体的分子遗传学分析
DOI：
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发表时间：
1997
影响因子：
4.8
通讯作者：
P. Fernandes
中科院分区：
文献类型：
--
作者：
P. M. Rose;J. Lynch;S. Frazier;S. Fisher;Wendy Chung;Peter B Battaglino;Z. Fathi;R. Leibel;P. Fernandes
神经肽Y是由36个氨基酸组成的肽酰胺，具有多种生物活性。这些功能是通过几种不同的受体介导的。到目前为止，已经克隆了五种受体亚型。在这里，我们报告了通过从下丘脑文库中低严格同源克隆，分离出编码鼠神经肽Y受体的人同源物的cDNA，该cDNA随后报道（1）。翻译的人Y1样受体克隆表明，它编码的受体是截短的第三个细胞外环。人Y1-样序列的人Y1受体的比较表明，截短的受体可能是由于在第六跨膜结构域中的单核苷酸缺失引起的移码。Southern杂交分析表明该基因在人类基因组中为单拷贝。该基因位于染色体5 q上。为了验证这一假设，即等位基因变异的核酸长度内的第六跨膜结构域的Y1样受体可能存在产生一个功能性受体，基因组DNA从192个不同年龄，种族背景，和程度的肥胖进行了分析，并通过直接测序。在任何受试者中均未检测到变异，表明该受体亚型可能是人类中转录的假基因。
Neuropeptide Y is a 36-amino-acid peptide amide with numerous biological activities. These functions are mediated through several pharmacologically distinct receptors. To date five receptor subtypes have been cloned. Here we report the isolation, by low stringency homology cloning from a hypothalamic library, of a cDNA encoding the human homolog of the murine neuropeptide Y receptor subsequently reported (1). Translation of the human Y1-like receptor clone suggested that it encoded a receptor which is truncated in the third extracellular loop. Comparison of the human Y1-like sequence to that of the human Y1 receptor suggested that the truncated receptor could have resulted from a frameshift due to a single nucleotide deletion in the sixth transmembrane domain. Southern blot analysis suggested that the gene is single copy in the human genome. The gene is located on chromosome 5q. To test the hypothesis that allelic variation of nucleic acid length within the sixth transmembrane domain of the Y1-like receptor may exist to produce a functional receptor, genomic DNA from 192 individuals of various ages, ethnic backgrounds, and degrees of obesity were analyzed electrophoretically and by direct sequencing. No variation was detected in any of the subjects, indicating that this receptor subtype may be a transcribed pseudogene in humans.
影响因子：
4.4
作者：
Trond Stokke;C. Collins;W. Kuo;David Kowbel;F. Shadravan;Minna Tanner;A. Kallioniemi;Olli Kallioniemi;D. Pinkel;D. Pinkel;L. L. Deaven-L.;J. Gray;Joe W. Gray
通讯作者：
Trond Stokke;C. Collins;W. Kuo;David Kowbel;F. Shadravan;Minna Tanner;A. Kallioniemi;Olli Kallioniemi;D. Pinkel;D. Pinkel;L. L. Deaven-L.;J. Gray;Joe W. Gray
DOI：
--
发表时间：
1989-05
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
B. O'dowd;M. Hnatowich;M. Caron;R. Lefkowitz;M. Bouvier
通讯作者：
B. O'dowd;M. Hnatowich;M. Caron;R. Lefkowitz;M. Bouvier