Influenza A virus infection of primary differentiated airway epithelial cell cultures derived from Syrian golden hamsters
Influenza A virus infection of primary differentiated airway epithelial cell cultures derived from Syrian golden hamsters
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DOI：
10.1016/j.virol.2006.06.024
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发表时间：
2006-10-10
期刊：
影响因子：
3.7
通讯作者：
Pekosz, Andrew
中科院分区：
文献类型：
--
作者：
Newby, Celeste M.;Rowe, Regina K.;Pekosz, Andrew
The ability of several different influenza A virus strains to infect and replicate in primary, differentiated airway epithelial cell cultures from Syrian golden hamsters was investigated. All virus strains tested replicated equivalently in the cultures and displayed a preference for infecting nonciliated cells. This tropism correlated with the expression of both alpha 2,3- and alpha 2,6-linked sialic acid on the nonciliated cells. In contrast, the ciliated cells did not have detectable alpha 2,6-linked sialic acid and expressed only low amounts of alpha 2,3-linked sialic acid. In contrast to clinical isolates, laboratory strains of influenza A virus infected a limited number of ciliated cells at late times post-infection. The presence of alpha 2,3- and alpha 2,6-linked sialic acid residues on the same cell type suggests that Syrian golden hamsters and differentiated airway epithelial cell cultures derived from hamsters may provide a system for studying the reassortment of influenza A virus strains which utilize different forms of sialic acid as a primary virus receptor. (c) 2006 Elsevier Inc. All rights reserved.
