Isolation of the Cell Wall
Isolation of the Cell Wall
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DOI：
10.1007/978-1-4939-6533-5_14
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发表时间：
2017-01-01
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影响因子：
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通讯作者：
Jamet, Elisabeth
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文献类型：
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作者：
Canut, Herve;Albenne, Cecile;Jamet, Elisabeth
本章描述了一种用于研究多糖和蛋白质的细胞壁纯化方法。植物原代细胞壁主要由多糖（质量90- 95%）和蛋白质（质量5- 10%）组成。在生长结束时，特化细胞可以合成由多糖（约65%）和木质素（约35%）组成的木质化次生壁。由于其组成，细胞壁是具有最高密度的细胞隔室，这一特性用于其纯化。它在植物发育和对环境约束的响应中起着关键作用。它主要用于食品和纺织工业以及生物能源的生产。所有这些特征和用途解释了为什么它作为一个真正的细胞隔室的研究是高度感兴趣的。所提出的纯化方法可以用于大量的材料，但也可以缩小到500毫克的新鲜材料。还提供了用于检查细胞壁制备质量的工具，如蛋白质分析和显微镜观察。
This chapter describes a method allowing the purification of the cell wall for studying both polysaccharides and proteins. The plant primary cell wall is mainly composed of polysaccharides (90-95 % in mass) and of proteins (5-10 %). At the end of growth, specialized cells may synthesize a lignified secondary wall composed of polysaccharides (about 65 %) and lignin (about 35 %). Due to its composition, the cell wall is the cellular compartment having the highest density and this property is used for its purification. It plays critical roles during plant development and in response to environmental constraints. It is largely used in the food and textile industries as well as for the production of bioenergy. All these characteristics and uses explain why its study as a true cell compartment is of high interest. The proposed method of purification can be used for large amount of material but can also be downscaled to 500 mg of fresh material. Tools for checking the quality of the cell wall preparation, such as protein analysis and microscopy observation, are also provided.