New cationic liposomes as vehicles of m-tetrahydroxyphenylchlorin in photodynamic therapy of infectious diseases
New cationic liposomes as vehicles of m-tetrahydroxyphenylchlorin in photodynamic therapy of infectious diseases
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DOI：
10.1021/mp800037d
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发表时间：
2008-07-01
影响因子：
4.9
通讯作者：
Venanzi, Mariano
中科院分区：
文献类型：
--
作者：
Bombelli, Cecilia;Bordi, Federico;Venanzi, Mariano
抗微生物光动力疗法正在成为细菌感染的一种有前途的治疗方式。为了优化光敏剂四羟基苯基二氢卟酚的抗菌活性，将其包封在由不同比例的二肉豆蔻酰-sn-甘油-磷脂酰胆碱和来自L-脯氨醇的四种阳离子表面活性剂中的任何一种组成的混合阳离子脂质体中。不同脂质体制剂的递送效率已经在耐甲氧西林金黄色葡萄球菌细菌菌株（MRSA）上进行了评价，并且所测试的制剂之一显示出与游离二氢卟酚相当的生物活性。为了使控制生物活性的载体的物理化学参数合理化，通过测量（a）zeta电位，（B）二氢卟酚包封效率的能力，即包封效率，（c）储存对二氢卟酚包封的影响和（d）二氢卟酚在双层中的定位来表征新的脂质体制剂。制剂的物理化学和生物学特征的相关性使我们能够在一定程度上合理化可能控制与生物环境相互作用的一些参数。
Antimicrobial photodynamic therapy is emerging as a promising therapeutic modality for bacterial infections. For optimizing the antibacterial activity of the photosensitizer in-tetrahydroxyphenylchlorin, it has been encapsulated in mixed cationic liposomes composed of different ratios of dimyristoyl-sn-glycero-phosphatidylcholine and any of four cationic surfactants derived from L-prolinol. The delivery efficiency of the different liposomes formulations has been Evaluated on a methicillin-resistant Staphylococcus aureus bacterial strain (MRSA), and one of the tested formulations shows a biological activity comparable to that of the free chlorin. In order to rationalize the physicochemical parameters of the carriers that control the biological activity, the new liposome formulations have been characterized by measuring (a) the zeta potential, (b) the r capability of chlorin entrapping efficiency, i.e. entrapment efficacy, (c) the effect of storage on chlorin entrapment and (d) the localization of chlorin in the bilayer. The correlation of the physicochemical and biological features of formulations has allowed us to rationalize, to some extent, some of the parameters that may control the interactions with the biological environment.