Analysis of the effector activity of Ara h 2 and Ara h 6 by selective depletion from a crude peanut extract.
Analysis of the effector activity of Ara h 2 and Ara h 6 by selective depletion from a crude peanut extract.
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DOI：
10.1016/j.jim.2011.06.031
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发表时间：
2011-09-30
影响因子：
2.2
通讯作者：
Dreskin, Stephen C.
中科院分区：
文献类型：
--
作者：
Chen, Xueni;Zhuang, Yonghua;Wang, Qian;Moutsoglou, Daphne;Ruiz, Glenn;Yen, S. -E.;Dreskin, Stephen C.
It is important to know the contribution of specific allergens to a complex allergenic extract and to have a dependable method to assess the effector activity of an extract specifically depleted of that allergen. We have previously shown that removal of the major peanut allergen, Ara h 2, from a crude peanut extract (CPE) minimally altered the effector activity of the extract. Here we describe in detail the methodology used to generate specific rabbit anti-peptide antibodies to remove a related peanut allergen, Ara h 6, from CPE and describe an improvement in the RBL SX-38 cell assay used to assess the effector activity of treated extracts. Our results show that although Ara h 2 and Ara h 6 can be selectively removed from a CPE, removal of each alone from a CPE had no significant effect on the effector activity. However, removal of Ara h 2 and Ara h 6 together significantly reduced the effector activity of CPE.
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影响因子：
14.2
作者：
DEGROOT, H;VANSWIETEN, P;AALBERSE, RC
通讯作者：
AALBERSE, RC
影响因子：
6.1
作者：
Ocmant, A.;Mulier, S.;Schandene, L.
通讯作者：
Schandene, L.
影响因子：
14.2
作者：
LOMBARDERO, M;QUIRCE, S;CARREIRA, J
通讯作者：
CARREIRA, J
影响因子：
6.1
作者：
Blanc, F.;Adel-Patient, K.;Bernard, H.
通讯作者：
Bernard, H.
DOI：
10.1111/j.1365-2222.2009.03273.x
发表时间：
2009-07
期刊：
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology
影响因子：
--
作者：
Porterfield HS;Murray KS;Schlichting DG;Chen X;Hansen KC;Duncan MW;Dreskin SC
通讯作者：
Dreskin SC