Origin of the zebrafish endocrine and exocrine pancreas.
Origin of the zebrafish endocrine and exocrine pancreas.
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斑马鱼内分泌和外分泌胰腺的起源。
DOI：
10.1002/dvdy.21168
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Prince,VictoriaE
中科院分区：
文献类型：
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作者：
Ward,AndreaB;Warga,RachelM;Prince,VictoriaE
在这里，我们报告了斑马鱼胰腺在原肠胚发育早期(受精后6小时；HPF)的详细命运图。我们证明，在这个阶段，胰腺和肝脏的前体细胞都对称地定位在相对于背部组织者的两个广泛的区域。我们证明，背侧和腹侧的胰腺芽可以来自6HPF的共同祖细胞池，但通常来自独立的群体。内分泌型和外分泌型胰腺表现出类似的祖细胞模式，与背芽严格内分泌和腹芽主要外分泌的描述一致。总体而言，我们发现内分泌/背芽祖细胞比外分泌胰腺/腹芽前体细胞位于更背侧。在原肠发育后期(10HPF)，胰腺祖细胞迁移到胚胎赤道的双侧区域。我们的命运图将有助于设计和解释未来的实验，以了解胰腺的早期发育。发展动力学236：1558-1569,2007。©2007 Wiley-Liss Inc.
Here, we report a detailed fate map of the zebrafish pancreas at the early gastrula stage of development (6 hours postfertilization; hpf). We show that, at this stage, both pancreas and liver progenitors are symmetrically localized in two broad domains relative to the dorsal organizer. We demonstrate that the dorsal and ventral pancreatic buds can derive from common progenitor pools at 6 hpf, but often derive from independent populations. Endocrine vs. exocrine pancreas show a similar pattern of progenitors, consistent with descriptions of the dorsal bud being strictly endocrine and the ventral bud primarily exocrine. In general, we find that endocrine/dorsal bud progenitors are located more dorsally than the exocrine pancreas/ventral bud progenitors. Later in gastrulation (10 hpf), pancreas progenitors have migrated to bilateral domains at the equator of the embryo. Our fate map will assist with design and interpretation of future experiments to understand early pancreas development. Developmental Dynamics 236:1558–1569, 2007. © 2007 Wiley‐Liss, Inc.