Protective effect of Jolkinolide B on LPS-induced mouse acute lung injury
Protective effect of Jolkinolide B on LPS-induced mouse acute lung injury
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Jolkinolide B对LPS诱导的小鼠急性肺损伤的保护作用
DOI：
10.1016/j.intimp.2015.03.021
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发表时间：
2015-05-01
影响因子：
5.6
通讯作者：
He, Chengyan
中科院分区：
文献类型：
--
作者：
Yang, Hailing;Li, Yan;He, Chengyan
Jolkinolide B （JB）是一种从大戟（Euphorbia fischeriana）干根中分离得到的对戊二烯二萜，具有较强的抗肿瘤和抗炎活性。然而，JB对急性肺损伤（ALI）的影响及其潜在的分子机制尚未被研究。本研究旨在探讨JB对脂多糖（LPS）诱导的ALI的影响。雄性C57BL/6小鼠经鼻灌胃LPS前1 h，分别用地塞米松或JB预处理。结果表明，JB能显著减轻lps诱导的组织学改变、肺水肿、炎症细胞浸润、髓过氧化物酶（MPO）活性以及tnf - α、IL-6和IL-1 β的产生。此外，JB还能显著抑制lps诱导的I κ B α降解。NF-kappa B p65和MAPK的磷酸化。因此，我们的研究提供了第一个证据，证明JB预处理对lps诱导的小鼠ALI具有保护作用。JB的抗炎机制可能与抑制NF-kappa B和MAPK的激活有关。(C) 2015 Elsevier B.V.版权所有
Jolkinolide B (JB), an ent-abietane diterpenoid, isolated from the dried root of Euphorbia fischeriana, has been reported to have potent anti-tumor and anti-inflammatory activities. However, the effects of JB on acute lung injury (ALI) and underlying molecular mechanisms have not been investigated. The present study aimed to investigate the effect of JB on lipopolysaccharide (LPS)-induced ALI. Male C57BL/6 mice were pretreated with dexamethasone or JB 1 h before intranasal instillation of LPS. The results showed that JB markedly attenuated LPS-induced histological alterations, lung edema, inflammatory cell infiltration, myeloperoxidase (MPO) activity as well as the production of TNE-alpha, IL-6 and IL-1 beta. Furthermore, JB also significantly inhibited LPS-induced the degradation of I kappa B alpha. and phosphorylation of NF-kappa B p65 and MAPK. Therefore, our study provides the first line of evidence that pretreatment of JB has a protective effect on LPS-induced ALI in mice. The anti-inflammatory mechanism of JB may be attributed to its suppression of NF-kappa B and MAPK activation. (C) 2015 Elsevier B.V. All rights reserved.