MURINE T-CELL RECEPTOR MUTANTS WITH DELETIONS OF BETA-CHAIN VARIABLE REGION GENES
MURINE T-CELL RECEPTOR MUTANTS WITH DELETIONS OF BETA-CHAIN VARIABLE REGION GENES
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DOI：
10.1073/pnas.83.3.767
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发表时间：
1986-02-01
影响因子：
11.1
通讯作者：
LOH, DY
中科院分区：
文献类型：
--
作者：
BEHLKE, MA;CHOU, HS;LOH, DY
对来自8个品系的小鼠的DNA进行基因组Southern印迹，检查其编码T细胞受体β可变区的基因座中的限制性片段长度多态性。链（V β），使用16种不同的V β-特异性探针小鼠品系BALB/c、C57 BL/6、C3 H和PL是相同的，而品系SJL、C57 BR、C57 L和SWR与其他四个品系共享若干多态性。此外，SJL、C57 L、C57 BR和SWR DNA在BALB/c DNA中缺失50%的杂交条带。构建来自伴刀豆球蛋白A刺激的SJL脾胚细胞的cDNA文库并检测V β。基因使用发现了10个基因来解释所有的V β-包含分离的克隆，包括三个新鉴定的V β。基因.所有10个这些基因被发现存在于BALB/c小鼠。我们得出结论，SJL、C57 L、C57 BR和SWR小鼠代表V β。BALB/c基因型的缺失突变体。
Genomic Southern blots of DNA from eight strains of mice were examined for restriction fragment length polymorphisms in their loci encoding the variable region of the T-cell receptor .beta. chain (V.beta.), using 16 different V.beta.-specific probes. Mouse strains BALB/c, C57BL/6, C3H, and PL were identical, while strains SJL, C57BR, C57L, and SWR shared several polymorphisms with respect to the other four strains. In addition, SJL, C57L, C57BR, and SWR DNAs were missing 50% of the hybridizing bands visualized in BALB/c DNA. A cDNA library from concanavalin A-stimulated SJL spleen blasts was constructed and examined for V.beta. gene usage. Ten genes were found to account for all V.beta.-containing clones isolated, including three newly identified V.beta. genes. All 10 of these genes were found to be present in BALB/c mice. We conclude that SJL, C57L, C57BR, and SWR mice represent V.beta. deletion mutants of the BALB/c genotype.