WILD-TYPE AND MUTANT ACTIN GENES IN CAENORHABDITIS-ELEGANS
WILD-TYPE AND MUTANT ACTIN GENES IN CAENORHABDITIS-ELEGANS
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DOI：
10.1016/0022-2836(89)90503-2
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发表时间：
1989-08-05
影响因子：
5.6
通讯作者：
HIRSH, D
中科院分区：
文献类型：
--
作者：
KRAUSE, M;WILD, M;HIRSH, D
我们对秀丽隐杆线虫的四个肌动蛋白基因进行了测序。这四个编码典型的无脊椎动物肌动蛋白，并且高度同源，彼此最多相差三个氨基酸残基。作为第一步，对这个基因集的发展调控的理解，我们也测序突变肌动蛋白基因。突变体基因克隆从一个单一的显性肌动蛋白突变体的两个独立的回复。对于这两种回复突变体，逆转伴随着肌动蛋白基因重排。肌动蛋白mRNA的积累过程中的发展，这两个回复是不同的野生型动物。我们在这里提出了肌动蛋白基因结构和野生型和突变动物的表达之间的相关性。结果表明，肌动蛋白基因的协调调节不是野生型肌肉功能所必需的。此外，至少一种肌动蛋白mRNA的3“区域的变化可能会影响其在发育过程中的稳态调节。
We have sequenced the four actin genes of Caenorhabditis elegans. These four encode typical invertebrate actins and are highly homologous, differing from each other by, at most, three amino acid residues. As a first step towards an understanding of the development regulation of this gene set we have also sequenced mutant actin genes. The mutant genes were cloned from two independent revertants of a single dominant actin mutant. For both revertants, reversion was accompanied by an actin gene rearrangement. The accumulation of actin mRNA during development in these two revertants is different from that of wild-type animals. We present here a correlation between actin gene structure and expression in wild-type and mutant animals. The results suggest that co-ordinate regulation of actin genes is not essential for wild-type muscle function. In addition, it appears that changes in the 3'' region of at least one of the actin mRNAs may affect its steady-state regulation during development.