In vivo effects of chronic treatment with [MET5]-enkephalin on hematological values and natural killer cell activity in athymic mice.
In vivo effects of chronic treatment with [MET5]-enkephalin on hematological values and natural killer cell activity in athymic mice.
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DOI：
10.1016/s0024-3205(99)00655-4
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发表时间：
2000-01
期刊：
影响因子：
6.1
通讯作者：
R. Zalys;I. Zagon;R. H. Bonneau;C. Lang;P. McLaughlin
中科院分区：
文献类型：
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作者：
R. Zalys;I. Zagon;R. H. Bonneau;C. Lang;P. McLaughlin
内源性阿片类物质在免疫机制中的作用通过使无胸腺（nu/nu）小鼠长期注射阿片类激动剂[Met 5]-脑啡肽（MET）或用纳洛酮（NTX）连续阻断阿片受体来检查。给药8天后，过量肽或受体阿片样物质剥夺对体重、脾指数（脾与体重比）、总白色血细胞计数和分类计数以及外周血或脾淋巴细胞中的自然杀伤（NK）细胞活性均无任何影响。在第28天，MET或NTX长期治疗对这些参数中的任何一个都没有影响，除了接受NTX的小鼠外周血中NK细胞活性相对于对照升高，以及MET组中与脾淋巴细胞相关的NK细胞活性低于正常。这些结果表明，长期暴露于阿片受体激动剂或持续性阿片受体阻断剂，对无胸腺小鼠的各种免疫学特性几乎没有影响，这表明天然阿片类药物，如MET，在无胸腺小鼠的防御机制中没有发挥显着的作用。
The role of endogenous opioids in immunological mechanisms was examined by subjecting athymic (nu/nu) mice to chronic injections of the opioid agonist [Met5]-enkephalin (MET) or continuous opioid receptor blockade with naltrexone (NTX). After 8 days of treatment, neither excess peptide nor deprivation of opioids from receptors had any effect on body weight, spleen index (spleen to body weight ratio), total and differential white blood cell counts, and natural killer (NK) cell activity in peripheral blood or splenic lymphocytes. At 28 days, chronic treatment with MET or NTX had no effect on any of these parameters with the exception of an elevation from controls in NK cell activity in peripheral blood in mice receiving NTX, and subnormal NK cell activity related to splenic lymphocytes in the MET group. These results suggest that chronic exposure to an opioid agonist, or persistent opioid receptor blockade, have little influence on a variety of immunological properties in athymic mice, suggesting that native opioids such as MET do not play a marked role in defense mechanisms in the athymic mouse.