RELATED TARGET ENHANCERS FOR DORSAL AND NF-KAPPA-B SIGNALING PATHWAYS
RELATED TARGET ENHANCERS FOR DORSAL AND NF-KAPPA-B SIGNALING PATHWAYS
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DOI：
10.1126/science.8146656
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发表时间：
1994-04-08
期刊：
影响因子：
56.9
通讯作者：
LEVINE, M
中科院分区：
文献类型：
--
作者：
GONZALEZCRESPO, S;LEVINE, M
果蝇背腹 (DV) 模式和哺乳动物造血受到相关信号通路（Toll、白介素-1）和转录因子（背侧、核因子-kappaB）的调节。这些因子与相关增强子相互作用，例如菱形 NEE 和 kappa 轻链增强子，它们包含类似的激活子和阻遏子结合位点排列。结果表明，kappa 增强子可以在转基因果蝇胚胎中产生基因表达的横向条纹，其模式类似于菱形 NEE 所指导的模式。果蝇 DV 决定簇通过 kappa 增强子中相应的哺乳动物顺式调控元件（包括 kappaB 位点和 kappaE 盒）引导这些条纹。这些结果表明增强子可以将保守的信号通路与不同的基因功能结合起来。
Drosophila dorsoventral (DV) patterning and mammalian hematopoiesis are regulated by related signaling pathways (Toll, interleukin-1) and transcription factors (dorsal, nuclear factor-kappaB). These factors interact with related enhancers, such as the rhomboid NEE and kappa light chain enhancer, that contain similar arrangements of activator and repressor binding sites. It is shown that the kappa enhancer can generate lateral stripes of gene expression in transgenic Drosophila embryos in a pattern similar to that directed by the rhomboid NEE. Drosophila DV determinants direct these stripes through the corresponding mammalian cis regulatory elements in the kappa enhancer, including the kappaB site and kappaE boxes. These results suggest that enhancers can couple conserved signaling pathways to divergent gene functions.