Biotechnology applied to fish reproduction: tools for conservation
Biotechnology applied to fish reproduction: tools for conservation
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DOI：
10.1007/s10695-018-0506-0
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发表时间：
2018-12-01
影响因子：
2.9
通讯作者：
Yasui, George Shigueki
中科院分区：
文献类型：
--
作者：
de Siqueira-Silva, Diogenes Henrique;Saito, Taiju;Yasui, George Shigueki
本文讨论了新的生物技术工具，出现和有前途的保护和提高鱼类生产，主要是关于濒危和最重要的经济物种。特别是，有两种主要技术可用于避免濒危鱼类物种灭绝和提高商业物种的产量。生殖细胞移植技术包括胚胎-胚胎卵裂球移植、胚胎-胚胎分化的PGC移植、幼虫-幼虫和胚胎分化的PGC移植、精原细胞成体-成体或成体间移植、卵原细胞移植等。然而，生殖细胞移植的成功依赖于预先对鱼进行绝育，这可以在鱼类发育的不同阶段通过几种已经过测试的方案进行，以实现生产无菌鱼的最佳方法。其中，鱼类杂交和三倍化，生殖系基因敲低，高温，和化学处理值得关注的基础上取得的重要成果迄今。本文综述了目前使用的替代技术和鱼类绝育的技术和知识，讨论了每种方法的优点和缺点。
This review discusses the new biotechnological tools that are arising and promising for conservation and enhancement of fish production, mainly regarding the endangered and the most economically important species. Two main techniques, in particular, are available to avoid extinction of endangered fish species and to improve the production of commercial species. Germ cell transplantation technology includes a number of approaches that have been studied, such as the transplantation of embryo-to-embryo blastomere, embryo-to-embryo differentiated PGC, larvae to larvae and embryo differentiated PGC, transplantation of spermatogonia from adult to larvae or between adults, and oogonia transplantation. However, the success of germ cell transplantation relies on the prior sterilization of fish, which can be performed at different stages of fish species development by means of several protocols that have been tested in order to achieve the best approach to produce a sterile fish. Among them, fish hybridization and triploidization, germline gene knockdown, hyperthermia, and chemical treatment deserve attention based on important results achieved thus far. This review currently used technologies and knowledge about surrogate technology and fish sterilization, discussing the stronger and the weaker points of each approach.